Noohi Nasrin, Papizadeh Moslem, Rohani Mahdi, Talebi Malihe, Pourshafie Mohammad R
Research Center for Conservation of Cultural Relics, Research Center of Iranian Cultural Heritage and Tourism Organization, Tehran, Iran.
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
Anim Nutr. 2021 Mar;7(1):119-126. doi: 10.1016/j.aninu.2020.07.005. Epub 2020 Dec 28.
Considering the importance of the poultry industry and the increasing interest in alternative growth promoters, probiotics are considered as a potential candidate for use in the poultry industry. In this study, species were isolated from 21 rectal swabs of 11 healthy 6-day-old and 10 healthy 21-day-old chickens and their fecal and feed samples. The isolates were characterized and their probiotic characteristics, including resistance to gastric acid and bile salts, biofilm formation and adherence to epithelium or mucus, amylase and protease activity and production of inhibitory compounds, were assessed. From 31 acid and bile resistant lactobacilli, only 2 and 1 strains showed significant probiotic properties. These isolates indicated detectable attachment to Caco-2 cells and significant antibacterial activities against Gram-positive and Gram-negative pathogens Additionally, phenotypic and genotypic diversity of lactobacilli isolates were studied by Phene Plate (PhP) system (PhP-LB) and random amplified polymorphic DNA (RAPD)-PCR, respectively. PhP-LB results of 24 isolates showed a high phenotypic variation among the isolates. In comparison, results of RAPD-PCR highlighted a low diversity. Therefore, it seems that combination of the 2 techniques (PhP and RAPD-PCR) could result in a significant discriminatory power than each of them used alone.
考虑到家禽业的重要性以及对替代生长促进剂的兴趣日益增加，益生菌被视为家禽业中一种潜在的候选物。在本研究中，从11只6日龄健康鸡和10只21日龄健康鸡的21份直肠拭子及其粪便和饲料样本中分离出菌株。对分离株进行了表征，并评估了它们的益生菌特性，包括对胃酸和胆汁盐的抗性、生物膜形成以及对上皮或黏液的黏附、淀粉酶和蛋白酶活性以及抑制性化合物的产生。在31株耐酸和耐胆汁的乳酸杆菌中，只有2株和1株表现出显著的益生菌特性。这些分离株显示出可检测到的对Caco-2细胞的附着以及对革兰氏阳性和革兰氏阴性病原体的显著抗菌活性。此外，分别通过Phene Plate（PhP）系统（PhP-LB）和随机扩增多态性DNA（RAPD）-PCR研究了乳酸杆菌分离株的表型和基因型多样性。24株分离株的PhP-LB结果显示分离株之间存在高度的表型变异。相比之下，RAPD-PCR结果突出显示多样性较低。因此，似乎这两种技术（PhP和RAPD-PCR）结合使用可能比单独使用其中任何一种技术具有更强的鉴别力。
