In Vitro Antibacterial and Antibiofilm Activities of L. Ethanolic Leaf Extract on ATCC 29213.

BACKGROUND AND OBJECTIVE

Staphylococcus aureus is the leading cause of skin and soft tissue infections such as abscesses, furuncles, and cellulitis. Biofilm forming strains of have higher incidence of antimicrobial resistance to at least three or more antibiotics and are considered as multidrug resistant. Since biofilm-producing strains have higher rates of multidrug and methicillin resistance compared to non-biofilm-producing strains, the need for alternative therapeutic option is important. Furthermore, rates of methicillin-resistant (MRSA) in Asia remain high. Results of the study may provide support for the clinical uses of as a topical antibacterial and antiseptic in the treatment and prevention of infections involving the skin, mouth, throat, and indwelling medical devices. Thus, this study aimed to evaluate the in vitro antibacterial and antibiofilm activities of L. ethanolic leaf extract (PBE) against a biofilm-forming methicillin-sensitive ATCC 29213 (MSSA).

METHODS

The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PBE against MSSA were determined using the agar dilution assay. The biofilm inhibition and eradication assays using crystal violet were done to quantify the antibiofilm activities of PBE on MSSA biofilm.

RESULTS

PBE showed activity against MSSA in agar dilution assay with MIC and MBC values of 2500 μg/mL and 5000 μg/mL, respectively. At subinhibitory concentrations, PBE showed biofilm inhibition activity at 1250 μg/mL but a lower percent eradication of biofilms as compared to oxacillin was noted.

CONCLUSION

PBE showed antibacterial activities including biofilm inhibition against methicillin-sensitive ATCC 29213 (MSSA).