Hanaoka Chad, Pichika Rajeswari, Dayanidhi Sudarshan, Jayabalan Prakash
Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Shirley Ryan AbilityLab, Chicago, IL, USA.
Dev Med Child Neurol. 2025 May;67(5):639-647. doi: 10.1111/dmcn.16105. Epub 2024 Oct 21.
To evaluate whether serum metabolomics differ between ambulatory individuals with cerebral palsy (CP) compared with individuals with typical development and whether functional capacity is associated with metabolite abundance.
Thirty-eight adolescents and young adults were enrolled (CP: n = 19; typical development: n = 19). After functional capacity testing (10-meter walk, sit-to-stand, and peak knee flexion/extension torques), blood was drawn. Targeted serum metabolomics on hydrophilic metabolites were performed by high-performance liquid chromatography coupled with high-resolution and tandem mass spectrometry. Metabolite dimensionality reduction, pathway analysis, fold change, and t-tests evaluated changes in metabolite abundance. Associations were tested between functional measures and metabolite abundance.
Individuals with CP had a significant increase in the abundance of essential amino acids, catabolic products of protein metabolism, and tricarboxylic acid cycle substrates, such as valine, tryptophan, kynurenic acid, and pyruvate (p < 0.05). Importantly, the abundance of numerous metabolites was only highly associated with functional capacity in individuals with CP such that greater abundance was associated with greater capacity, but not in those with typical development.
Our findings show clear increases in serum metabolites in individuals with CP, which are associated with functional capacity for movement. The altered metabolite profile measured after exercise might reflect increased energy production needed for movement. Appropriate nutritional intake during exercise might be needed given increased energy requirements.
评估与发育正常个体相比，非卧床脑瘫（CP）个体的血清代谢组学是否存在差异，以及功能能力是否与代谢物丰度相关。
招募了38名青少年和青年（CP组：n = 19；发育正常组：n = 19）。在进行功能能力测试（10米步行、坐立试验以及膝关节屈伸峰值扭矩测试）后采集血液。采用高效液相色谱联用高分辨率串联质谱法对亲水性代谢物进行靶向血清代谢组学分析。通过代谢物降维、通路分析、倍数变化和t检验评估代谢物丰度的变化。测试功能指标与代谢物丰度之间的相关性。
CP个体的必需氨基酸、蛋白质代谢分解产物以及三羧酸循环底物（如缬氨酸、色氨酸、犬尿烯酸和丙酮酸）的丰度显著增加（p < 0.05）。重要的是，许多代谢物的丰度仅与CP个体的功能能力高度相关，即丰度越高，能力越强，但在发育正常个体中并非如此。
我们的研究结果表明，CP个体的血清代谢物明显增加，这与运动功能能力相关。运动后测得的代谢物谱改变可能反映了运动所需能量产生的增加。鉴于能量需求增加，运动期间可能需要适当的营养摄入。
