Kim Dong Hyun, Seo Hyejun, Jung Sangkwon, Kim Bum-Joon
Department of Microbiology and Immunology, College of Medicine, Seoul National University, Seoul, South Korea.
Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul, South Korea.
Microbiol Spectr. 2024 Oct 21;12(12):e0193524. doi: 10.1128/spectrum.01935-24.
is a group of emerging antimicrobial-resistant nontuberculous mycobacteria that causes severe lung disease in infected patients globally. Recently, molecular epidemiology studies have indicated that horizontal gene transfer (HGT) events in the gene are prevalent between subspecies. To determine the global prevalence of strains subjected to HGT, we performed phylogenetic inference using a 711-bp sequence extracted from 1,786 . isolates for which the whole-genome sequence was publicly available. Our data showed that a total of 74 isolates (4.1%) from 1,786 strains are subject to HGT, which is more prevalent than strains with HGT (19 isolates from 1,786, 1.1%). Most of these (69 isolates) belong to two major groups of , of which the gene is horizontally transferred from (Rec-mas), dominant circulating clone 7 (DCC7) (44 isolates) and ST46 type by multilocus sequence typing (25 isolates). The Rec-mas strains of the two groups have distinct geographical patient distributions, of which the former is mainly distributed in the United States, while the latter is prevalent in Asia. Our further genome-based analysis indicated that the ST46 type is a novel DCC candidate of that is responsible for dissemination between noncystic fibrosis patients in Asia. In conclusion, our global phylogenetic analysis revealed two major Rec-mas clones with distinct geographical distributions, namely, DCC7 and ST46. This study provides insights into the genetic clustering and person-to-person transmission of globally dominant and area-specific strains harboring the HGT gene.
Horizontal gene transfer (HGT) events play a pivotal role in the evolution of into dominant circulating clones (DCCs), which is capable of causing patient-to-patient transmission. In particular, HGT of the rpoB gene between strains of different subspecies of could also compromise differentiation between strains of . Here, for the first time, using 1,786 genome sequences, we evaluated the global prevalence of strains subjected to HGT. We found a greater prevalence of subjected to HGT than to those subjected to HGT, which is mainly due to two Rec-mas clones, dominant circulating clone 7 and ST46, which are responsible for dissemination between non-CF patients in Asia. Our data highlight the importance of HGT in the evolution of , particularly , into virulent DCC clones.
是一组新出现的耐抗菌性非结核分枝杆菌，在全球感染患者中引起严重肺部疾病。最近，分子流行病学研究表明，该基因中的水平基因转移（HGT）事件在亚种之间普遍存在。为了确定经历HGT的菌株的全球流行情况，我们使用从1786株全基因组序列公开可用的菌株中提取的711bp序列进行了系统发育推断。我们的数据显示，1786株菌株中共有74株（4.1%）经历了HGT，这比经历HGT的菌株（1786株中的19株，1.1%）更普遍。其中大多数（69株）属于两个主要的菌株组，其基因是从（Rec-mas）、优势循环克隆7（DCC7）（44株）和多位点序列分型的ST46型水平转移而来（25株）。这两组的Rec-mas菌株具有不同的地理患者分布，其中前者主要分布在美国，而后者在亚洲流行。我们进一步基于基因组的分析表明，ST46型是一种新的DCC候选菌株，负责在亚洲非囊性纤维化患者之间传播。总之，我们的全球系统发育分析揭示了两个具有不同地理分布的主要Rec-mas克隆，即DCC7和ST46。本研究为携带HGT基因的全球优势菌株和区域特异性菌株的遗传聚类和人际传播提供了见解。
水平基因转移（HGT）事件在演变为优势循环克隆（DCC）的过程中起关键作用，DCC能够导致患者之间的传播。特别是，不同亚种菌株之间的rpoB基因HGT也可能损害菌株之间的分化。在这里，我们首次使用1786株全基因组序列评估了经历HGT的菌株的全球流行情况。我们发现经历HGT的菌株比经历HGT的菌株更普遍，这主要是由于两个Rec-mas克隆，优势循环克隆7和ST46，它们负责在亚洲非CF患者之间传播。我们的数据突出了HGT在菌株进化中的重要性，特别是在演变为毒性DCC克隆方面。
