Centre Paul Pascal, UMR 5031, CNRS, avenue Schweitzer, 33600 Pessac, France.
Nano Lett. 2024 Oct 30;24(43):13481-13486. doi: 10.1021/acs.nanolett.4c02302. Epub 2024 Oct 21.
DNA nanopores appear to be a plausible alternative to the use of transmembrane proteins. The specificity and programmability of DNA interactions allow the design of synthetic channels that insert into lipid bilayers and can regulate the ionic transport across them. In this Communication, we investigate the dependence of insertion capabilities on the electrostatic properties of the nanopore and show that the presence of a permanent electric dipole is an important factor for the nanopore to insert into the membrane. On the contrary, in the absence of such a dipole, most DNA nanopores bind to the bilayer without channel formation. We also show that this modification does not hinder the possibility of triggering a measurable conformational change with a single short oligonucleotide.
DNA 纳米孔似乎是替代跨膜蛋白的一种合理选择。DNA 相互作用的特异性和可编程性允许设计插入脂质双层的合成通道，并可以调节它们的离子传输。在本通讯中，我们研究了插入能力对纳米孔静电特性的依赖性，并表明永久偶极子的存在是纳米孔插入膜的一个重要因素。相反，在没有这种偶极子的情况下，大多数 DNA 纳米孔与双层结合但不形成通道。我们还表明，这种修饰不影响使用单个短寡核苷酸触发可测量构象变化的可能性。
