Hassan Nermine, Riad Mona, Ibrahim Shereen Hafez, Mahmoud Khaled, Abulnoor Bassam Ahmed, Hassan Reham
Lecturer of Endodontics, Faculty of Dentistry, Cairo University, Giza, Egypt.
Professor of Conservative Dentistry, Faculty of Dentistry, Cairo University, Giza, Egypt.
BDJ Open. 2024 Oct 22;10(1):81. doi: 10.1038/s41405-024-00266-9.
Enhancing the antibacterial capabilities of dental materials by adding nanoparticles has been the subject of some research. However, the potential toxic effect of this material on the vital tissues should be investigated to avoid additional damage to the tissue.
This study aimed to validate the long-term cytotoxic and antibacterial properties of an epoxy resin-based endodontic sealer (AH Plus) with and without loading with silver gold nanoparticles (Nano Care Plus Silver Gold®).
The tested groups were Nano Care Gold (group I), modified resin sealer (m AH Plus; group II) and AH Plus served as a control group (group III). Agar diffusion was used to measure the antibacterial activity against Enterococcus faecalis. Using the MTT test, cytotoxicity assessment was carried out in accordance with ISO-10993-5 guidelines to assess the cells' viability as soon as possible and after two and four weeks. The t-test was used to statistically examine the data. The chosen significance threshold was P <0.05.
Antibacterial results revealed that there was no difference in the diameter of the inhibition zones measured in all groups at 24 h. While in 48 and 72 h, the difference was statistically significant (p <0.05). In 48-h Nanogold was significantly higher than AH Plus when tested alone (p <0.05), however their mixture showed insignificant difference. After 72 h, the Nano gold was significantly higher than that of AH Plus & Nano gold mixture (p <0.05). Cytotoxicity result revealed there was a significant difference between tested groups at different intervals (p <0.001). For immediate measurements, values measured with the AH group were significantly higher than those of other groups (p <0.001). For the AH& nanogold group, there was no significant difference between values measured at different intervals (p = 0.578).
Silver gold nanoparticles have acceptable antibacterial properties and low cytotoxicity to be used as canal pretreatment prior to the application of the sealer or even incorporated with AH Plus sealer.
通过添加纳米颗粒来增强牙科材料的抗菌能力已成为一些研究的主题。然而，这种材料对重要组织的潜在毒性作用应进行研究，以避免对组织造成额外损伤。
本研究旨在验证含和不含银金纳米颗粒（Nano Care Plus Silver Gold®）的环氧树脂类根管封闭剂（AH Plus）的长期细胞毒性和抗菌性能。
测试组为纳米金组（I组）、改性树脂封闭剂（m AH Plus；II组），AH Plus作为对照组（III组）。采用琼脂扩散法测定对粪肠球菌的抗菌活性。使用MTT试验，根据ISO-10993-5指南进行细胞毒性评估，以尽快以及在两周和四周后评估细胞活力。采用t检验对数据进行统计学分析。选定的显著性阈值为P <0.05。
抗菌结果显示，所有组在24小时时测量的抑菌圈直径无差异。而在48小时和72小时时，差异具有统计学意义（p <0.05）。在48小时时，单独测试时纳米金明显高于AH Plus（p <0.05），但其混合物显示无显著差异。72小时后，纳米金明显高于AH Plus和纳米金混合物（p <0.05）。细胞毒性结果显示，测试组在不同时间间隔存在显著差异（p <0.001）。对于即时测量，AH组测量的值明显高于其他组（p <0.001）。对于AH和纳米金组，不同时间间隔测量的值之间无显著差异（p = 0.578）。
银金纳米颗粒具有可接受的抗菌性能和低细胞毒性，可在应用封闭剂之前用作根管预处理，甚至与AH Plus封闭剂混合使用。
