紫柄假丝酵母发育缺陷型化学信号突变体的分析。
Analysis of developmentally defective chemical signaling mutants of Polysphondylium violaceum.
作者信息
Ellsaesser C F, Kuhn P E, Hanna M H
出版信息
J Bacteriol. 1986 Feb;165(2):647-9. doi: 10.1128/jb.165.2.647-649.1986.
Abstract
Six aggregation-defective mutants of Polysphondylium violaceum dependent on external addition of the pheromone D factor for aggregation were isolated after nitrosoguanidine mutagenesis. With a screening technique based on synergistic development, D-factor-dependent mutants can be separated from other kinds of aggregateless mutants. Genetic complementation analyses of the newly isolated mutants showed them to be mutant at the aggA locus. Individual mutants exhibited different sensitivities to D factor(s), responding maximally over a 300-fold range of concentrations.
摘要
经亚硝基胍诱变后，分离出了六个多孢黏菌聚集缺陷型突变体，它们的聚集依赖于外源添加信息素D因子。通过基于协同发育的筛选技术，可将依赖D因子的突变体与其他类型的无聚集突变体区分开来。对新分离出的突变体进行的遗传互补分析表明，它们在aggA位点发生了突变。各个突变体对D因子表现出不同的敏感性，在300倍的浓度范围内反应最为强烈。
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