Warren A J, Warren W D, Cox E C
Genetics. 1976 May;83(1):25-47. doi: 10.1093/genetics/83.1.25.
A system for genetic analysis in the cellular slime mold P. violaceum has been developed. Two growth-temperature-sensitive mutants were isolated in a haploid strain and used to select rare diploid heterozygotes arising by spontaneous fusion of the haploid cells. A recessive mutation to cycloheximide resistance in one stain enables selection of segregants, which often appear to be aneuploid.-Aggregation-defective (ag-) mutants having a wide range of phenotypes were isolated in both temperature-sensitive strains after nitrosoguanidine treatment, and complementation tests were performed between pairs of these mutants. Of 380 diploids isolated, 32 showed defective aggregation and were considered to contain 2 noncomplenting ag- mutations. Among noncomplementing mutants interallelic complentation is common. Noncomplementing mutants fall into 4 complementation groups, and those within each complementation group are phenotypically similar. Statistical analysis of the results suggests that the number of complementation units involved in aggregation is about 50.
已经开发出一种用于在细胞黏菌紫芝中进行遗传分析的系统。在一个单倍体菌株中分离出两个生长温度敏感突变体，并用于选择由单倍体细胞自发融合产生的罕见二倍体杂合子。一个菌株中对环己酰亚胺抗性的隐性突变使得能够选择分离子，这些分离子通常似乎是非整倍体。在亚硝基胍处理后，在两个温度敏感菌株中都分离出了具有广泛表型的聚集缺陷（ag-）突变体，并对这些突变体进行了成对互补测试。在分离出的380个二倍体中，32个表现出聚集缺陷，被认为含有2个不互补的ag-突变。在不互补的突变体中，等位基因间互补很常见。不互补的突变体分为4个互补组，每个互补组内的突变体表型相似。结果的统计分析表明，参与聚集的互补单位数量约为50个。
