SPYGEN, Savoie Technolac-BP 274, Le Bourget-du-Lac, France.
PLoS One. 2011;6(8):e23398. doi: 10.1371/journal.pone.0023398. Epub 2011 Aug 8.
The precise knowledge of species distribution is a key step in conservation biology. However, species detection can be extremely difficult in many environments, specific life stages and in populations at very low density. The aim of this study was to improve the knowledge on DNA persistence in water in order to confirm the presence of the focus species in freshwater ecosystems. Aquatic vertebrates (fish: Siberian sturgeon and amphibian: Bullfrog tadpoles) were used as target species. In control conditions (tanks) and in the field (ponds), the DNA detectability decreases with time after the removal of the species source of DNA. DNA was detectable for less than one month in both conditions. The density of individuals also influences the dynamics of DNA detectability in water samples. The dynamics of detectability reflects the persistence of DNA fragments in freshwater ecosystems. The short time persistence of detectable amounts of DNA opens perspectives in conservation biology, by allowing access to the presence or absence of species e.g. rare, secretive, potentially invasive, or at low density. This knowledge of DNA persistence will greatly influence planning of biodiversity inventories and biosecurity surveys.
物种分布的精确知识是保护生物学的关键步骤。然而，在许多环境、特定生命阶段和种群密度非常低的情况下，物种检测可能极其困难。本研究旨在提高对水中 DNA 持久性的认识，以确认淡水生态系统中目标物种的存在。水生脊椎动物（鱼类：西伯利亚鲟鱼和两栖类：牛蛙蝌蚪）被用作目标物种。在对照条件（水箱）和野外条件（池塘）下，在去除 DNA 来源物种后，DNA 的可检测性随时间的推移而降低。在这两种情况下，DNA 的可检测性都不到一个月。个体密度也会影响水样中 DNA 可检测性的动态。可检测性的动态反映了 DNA 片段在淡水生态系统中的持久性。可检测到的 DNA 数量的短时间持久性为保护生物学开辟了新的前景，例如可以了解稀有、隐蔽、潜在入侵或密度低的物种的存在或缺失。对 DNA 持久性的了解将极大地影响生物多样性清查和生物安保调查的规划。
