Bhardwaj Ragini, Gawade Bharat H, Pathania Pooja, Talukdar Akshay, Kumar Prakash, Khan Suphiya, Singh Gyanendra Pratap
ICAR-National Bureau of Plant Genetic Resources, New Delhi, India.
Department of Bioscience and Biotechnology, Banasthali Vidyapith University, Vanasthali, India.
Front Genet. 2024 Oct 10;15:1482956. doi: 10.3389/fgene.2024.1482956. eCollection 2024.
Mungbean plays a significant role in global food and nutritional security. However, the recent drastic rise in atmospheric temperature has posed an imminent threat to mungbean cultivation. Therefore, this study investigates the growth and physiological changes of 87 mungbean germplasm lines under heat stress. Genotypes were examined using parameters including leaf area, chlorophyll content, membrane stability index (MSI), stomatal conductance, pollen viability, number of pods per cluster, number of pods per plant, number of seeds/pod, 100-seed weight and grain yield/plant under heat stress and control environments. A wide range of variation was observed for these traits among genotypes under heat stress and control environments. Genotypes were also identified with variable responses under both environments. The phenotypic expression of selected promising accessions was also validated in control environment conditions at the National Phytotron facility. The selected promising genotypes viz., IC76475, IC418452 and IC489062 validated their heat tolerance behavior for key candidate genes revealed by quantitative real-time PCR (qRT-PCR). These mungbean genotypes can act as potential resources in the mungbean improvement programs for heat stress tolerance. This study also provides a comprehensive understanding of the key mechanisms underlying heat tolerance in mungbean.
绿豆在全球粮食和营养安全方面发挥着重要作用。然而，近期大气温度的急剧上升对绿豆种植构成了迫在眉睫的威胁。因此，本研究调查了87个绿豆种质系在热胁迫下的生长和生理变化。利用包括叶面积、叶绿素含量、膜稳定性指数（MSI）、气孔导度、花粉活力、每簇荚数、每株荚数、每荚种子数、百粒重和单株籽粒产量等参数，在热胁迫和对照环境下对基因型进行了检测。在热胁迫和对照环境下，这些性状在基因型之间观察到广泛的变异。还鉴定出在两种环境下具有不同反应的基因型。在国家人工气候室的对照环境条件下，也验证了所选有前景种质的表型表达。所选有前景的基因型，即IC76475、IC418452和IC489062，通过定量实时PCR（qRT-PCR）验证了其对关键候选基因的耐热行为。这些绿豆基因型可作为绿豆耐热性改良计划中的潜在资源。本研究还全面了解了绿豆耐热性的关键机制。
