extract extends the lifespan and health-span of mitogen-activated protein kinase 1.

Aging is a highly complex process and one of the largest risk factors for many chronic diseases. (AM) is rich in bioactive phytochemicals with antioxidant, anti-inflammatory, and anticancer properties. However, little is known about its effects on aging. The objective of this study was to evaluate the effects of AM extract on lifespan and health-span using as a representative model. The mechanisms of its effects were explored using transcriptomics and untargeted metabolomics. Results showed that the lifespan of was significantly extended by 22.2% after high-dose AM treatment. AM improved the behavior and physiological functions of by increasing the pharyngeal pumping rate, decreasing lipofuscin accumulation and the reactive oxygen species level, enhancing resistance to oxidative stress, and increasing the activities of superoxide dismutase and catalase. Transcriptome analysis showed that the gene (mitogen-activated protein kinase 1), which is involved in the MAPK signaling pathway, was the gene with the largest fold change after AM intervention. However, in the mutant, the beneficial effect of AM in improving nematode senescence disappeared. An untargeted metabolomics study showed that the levels of 4-hydroxyproline, rhamnose, and cysteine were increased after AM supplementation, and their extending effect on the lifespan and health-span of were partly dependent on the gene. In conclusion, our results revealed that AM can promote the lifespan and health-span of the PMK-1 pathway, highlighting the potential of AM as a dietary supplement to delay aging.