Key Laboratory of Bio-Based Materials Science & Technology (Ministry of Education), College of Materials Science and Engineering, Northeast Forestry University, Harbin 150040, China.
School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China.
Biosensors (Basel). 2024 Oct 1;14(10):472. doi: 10.3390/bios14100472.
Detecting circulating tumor cells has exhibited great significance in treating cancers since its concentration is an index strongly associated with the development and transfer of the tumor. However, the present commercial method for CTC detection is still expensive, because special antibodies and complicated devices must be used for cell separation and imaging. Hence, it is quite necessary to apply alternative materials and methods to decrease the cost of CTC detection. In this article, we coated a cellulose acetate membrane with nanoparticles formed by the polymerization of melamine and furfural, creating a surface with nanoscale roughness for the highly efficient capture of the sparse CTCs in a blood sample. Subsequently, the CTCs on the surface can be quantitatively detected by colorimetry with the aid of a COF-based nanozyme. The detection limit (LOD) can be as low as 3 cells/mL, which is the lowest LOD among the colorimetric methods to our knowledge. Considering the low cost of fabricating the membrane for CTC capture and the robustness of nanozymes compared with natural enzymes, this CTC detection approach displays great potential to decrease the financial burden of commercial CTC detection.
检测循环肿瘤细胞（CTC）在癌症治疗中具有重要意义，因为其浓度是与肿瘤发展和转移密切相关的指标。然而，目前用于 CTC 检测的商业方法仍然昂贵，因为细胞分离和成像需要特殊的抗体和复杂的设备。因此，有必要应用替代材料和方法来降低 CTC 检测的成本。在本文中，我们用三聚氰胺和糠醛聚合形成的纳米颗粒涂覆醋酸纤维素膜，在表面形成纳米级粗糙度，以高效捕获血液样本中稀疏的 CTC。随后，借助基于 COF 的纳米酶通过比色法对表面上的 CTC 进行定量检测。检测限（LOD）低至 3 个细胞/ml，这是我们所知的比色法中最低的 LOD。考虑到用于 CTC 捕获的膜的低成本以及纳米酶相对于天然酶的稳健性，这种 CTC 检测方法有望降低商业 CTC 检测的经济负担。
