Xiong Zhiwei, Zhang Wanshan, Yin Hui, Wan Jiaxing, Wu Zhuozhuo, Gao Yuxia
National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou 341000, China.
Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants (2024SSY04181), Ganzhou 341000, China.
Biology (Basel). 2024 Oct 14;13(10):822. doi: 10.3390/biology13100822.
genes are crucial components of the effector-triggered immunity (ETI) system, responsible for recognizing pathogens and initiating immune responses. Although genes in many plant species have been extensively studied, the diversity of genes in remains largely unknown. Our analysis revealed significant variations in the copy numbers of genes among these species. Gene duplication and recombination were identified as the major driving forces behind this diversity. Additionally, horizontal gene transfer (HGT) emerged as the principal mechanism responsible for the increase in gene copy number in . The genes were classified into four categories: TIR-NBS-LRR (TNL), CC-NBS-LRR (CNL), RPW8-NBS-LRR (RNL), and NL. Our findings indicate that TNL, RNL, and CNL genes originated from NL genes through the acquisition of TIR and RPW8 domains, along with CC motifs, followed by the random loss of corresponding domains. Phylogenetic analysis suggested that genes originated alongside the species and underwent adaptive evolution, potentially playing crucial roles in the global colonization of . This study provides important insights into the diversity of genes and serves as a foundational dataset for future research aimed at breeding disease-resistant varieties.
基因是效应子触发免疫（ETI）系统的关键组成部分，负责识别病原体并启动免疫反应。尽管许多植物物种中的基因已得到广泛研究，但[某植物名称]中基因的多样性在很大程度上仍不为人知。我们的分析揭示了这些物种中基因拷贝数的显著差异。基因复制和重组被确定为这种多样性背后的主要驱动力。此外，水平基因转移（HGT）成为导致[某植物名称]中基因拷贝数增加的主要机制。这些基因被分为四类：TIR-NBS-LRR（TNL）、CC-NBS-LRR（CNL）、RPW8-NBS-LRR（RNL）和NL。我们的研究结果表明，TNL、RNL和CNL基因通过获得TIR和RPW8结构域以及CC基序，从NL基因起源，随后相应结构域随机丢失。系统发育分析表明，基因与该物种一同起源并经历了适应性进化，可能在[某植物名称]的全球定殖中发挥关键作用。本研究为基因的多样性提供了重要见解，并作为未来培育抗病[某植物名称]品种研究的基础数据集。
