Kiewra Dorota, Dyczko Dagmara, Žákovská Alena, Nejezchlebova Helena
Department of Microbial Ecology and Acaroentomology, University of Wrocław, 51-148 Wrocław, Poland.
Institute of Experimental Biology, Faculty of Science, Masaryk University, 60200 Brno, Czech Republic.
Insects. 2024 Oct 9;15(10):785. doi: 10.3390/insects15100785.
(1) Background: is responsible for the spreading of medically important pathogens. Monitoring the level of tick infection in various areas is essential for determining the potential tick-born risk. This study aimed to detect spp. and spp. in ticks collected in urban and protected areas both in Poland and the Czech Republic. (2) Methods: Ticks were collected by flagging in the years 2016-2017. spp. was detected using nested PCR targeting the gene and spp. using nested PCR targeting . (3) Results: In total, DNA of spp. was detected in 25.9% of samples. Ticks collected in Poland were more infected compared to the Czech Republic and ticks collected in protected areas were more infected with spp. than ticks collected in urban areas. The RFLP analysis showed the occurrence of and in both countries, and additionally , s.s., and in Poland. spp. was detected in 17.4% of , with comparable infection level in both countries; however, regional differences were observed. (4) Conclusion: The regional differences in spp. and spp. prevalence in indicate the complexity of factors influencing the level of infection and underline the need for adaptation public health surveillance strategies in each region.
(1) 背景： 负责传播重要医学病原体。监测各地区蜱虫感染水平对于确定潜在的蜱传疾病风险至关重要。本研究旨在检测在波兰和捷克共和国的城市和保护区收集的蜱虫中的 属和 属。(2) 方法：在2016 - 2017年通过拖旗法收集蜱虫。使用针对 基因的巢式PCR检测 属，使用针对 的巢式PCR检测 属。(3) 结果：总共在25.9%的样本中检测到 属的DNA。与捷克共和国相比，在波兰收集的蜱虫感染率更高，并且在保护区收集的蜱虫比在城市地区收集的蜱虫 属感染率更高。RFLP分析表明在两个国家都存在 和 ，此外在波兰还存在 、狭义 属和 。在17.4%的 中检测到 属，两国感染水平相当；然而，观察到区域差异。(4) 结论： 属和 属在 中的流行率存在区域差异，这表明影响感染水平的因素具有复杂性，并强调需要在每个地区调整公共卫生监测策略。
