Santos-Silva Thamires, Viana Inácio Silva, Queiroz Andrea Barros Piazzon S, de Oliveira Fabrício Singaretti, Horvath-Pereira Bianca de Oliveira, da Silva-Júnior Leandro Norberto, Araujo Michelle Silva, Canola Paulo Alescio, Dias Luís Gustavo Gosuen G, Soares Marcelo Melo, Miglino Maria Angelica
Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, SP, Brazil.
Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal Campus 14884-900, SP, Brazil.
J Funct Biomater. 2024 Oct 20;15(10):311. doi: 10.3390/jfb15100311.
Bone defects in animals can arise from various causes, including diseases, neoplasms, and most commonly, trauma. Comminuted fractures that exceed the critical size may heal poorly due to deficient or interrupted vascularization, resulting in an insufficient number of progenitor cells necessary for bone regeneration. In this context, 3D printing techniques using poly-L-lactic acid/graphene oxide (PLLA/GO) aim to address this issue by creating customized scaffolds combined with canine placenta hydrogel and mesenchymal stem cells for use in goat mandibles, compared to a control group using titanium plate fixation. Ten canine placentas were decellularized and characterized using histological techniques. A hydrogel derived from the canine placenta extracellular matrix (cpECM) was produced to improve cell attachment to the scaffolds. In vitro cytotoxicity and cell adhesion to the cpECM hydrogel were assessed by scanning electron microscopy (SEM). The resulting biomaterials, cpECM hydrogel and PLLA/GO scaffolds, maintained their functional structure and supported cell adhesion, maintenance, and proliferation in vitro. Thermography showed that PLLA/GO scaffolds with cpECM hydrogel performed effectively, similar to the control group. Computed tomography scans revealed bone calluses, suggesting an ongoing repair process. These findings demonstrate the innovative technological potential of these materials for use in surgical interventions. Future studies on PLLA/GO scaffolds will provide further insights into their effects on goat models.
动物的骨缺损可由多种原因引起，包括疾病、肿瘤，最常见的是创伤。超过临界尺寸的粉碎性骨折可能因血管化不足或中断而愈合不良，导致骨再生所需的祖细胞数量不足。在这种情况下，使用聚-L-乳酸/氧化石墨烯（PLLA/GO）的3D打印技术旨在通过创建定制支架来解决这个问题，该支架与犬胎盘水凝胶和间充质干细胞结合用于山羊下颌骨，与使用钛板固定的对照组相比。对10个犬胎盘进行脱细胞处理并使用组织学技术进行表征。制备了一种源自犬胎盘细胞外基质（cpECM）的水凝胶，以改善细胞与支架的附着。通过扫描电子显微镜（SEM）评估cpECM水凝胶的体外细胞毒性和细胞粘附。所得生物材料cpECM水凝胶和PLLA/GO支架保持了其功能结构，并在体外支持细胞粘附、维持和增殖。热成像显示，带有cpECM水凝胶的PLLA/GO支架效果良好，与对照组相似。计算机断层扫描显示有骨痂形成，表明正在进行修复过程。这些发现证明了这些材料在手术干预中的创新技术潜力。未来对PLLA/GO支架的研究将进一步深入了解其对山羊模型的影响。
