Clinical Identification of Two Novel -like Species Isolated as Pathogens of Granulomatous Lobular Mastitis.

Granulomatous lobular mastitis (GLM) is a rare benign breast inflammatory disease that affects women of childbearing age. species, especially , was reported as the pathogen of GLM. A recent study showed that the complex is composed of and two novel species, and . The study presents seven -like strains isolated from GLM patients. However, they turned out to be six strains of and one strain of according to 16sRNA sequencing. In order to conduct a phylogenetic study, we further sequenced the fusA and genes, which were frequently employed in studies of species. Novel Mass Spectral Peaks (MSPs) for were created with Bruker MALDI-TOF MS. Then, the identification power of the MSPs was tested by strains and remotely related spp. The antibiotic sensitivity tests were performed according to the CLSI M45 guidelines. All of the strains were not resistant to -lactams, vancomycin or linezolid. However, applying erythromycin and clindamycin could be fruitless. Phenotypic identification using a Vitek2 ANC ID card proved all of the strains were identified as . The test of Ala-Phe-Pro arylamidase and urease could be employed as the characteristics to distinguish from . Here, we present the identification, antibiotic sensitivity tests (ASTs) and epidemiological investigation of two novel -like species with the purpose of improving the understanding of -like species and related diseases.