Chávez-Avilés Mauricio Nahuam, García-Álvarez Margarita, Ávila-Oviedo José Luis, Hernández-Hernández Irving, Bautista-Ortega Paula Itzel, Macías-Rodríguez Lourdes Iveth
Laboratorio de Bioquímica y Biología Molecular, División de Ingeniería Bioquímica, Tecnológico Nacional de México/ITS de Ciudad Hidalgo, Hidalgo 61100, Mexico.
Laboratorio de Bioquímica Ecológica, Instituto de Investigaciones Químico-Biológicas, Morelia 58030, Mexico.
Microorganisms. 2024 Oct 3;12(10):2007. doi: 10.3390/microorganisms12102007.
Managing plant diseases caused by phytopathogenic fungi, such as anthracnose caused by species, is challenging. Different methods have been used to identify compounds with antibiotic properties. strains are a source of novel molecules with antifungal properties, including volatile organic compounds (VOCs), whose production is influenced by the nutrient content of the medium. In this study, we assessed the VOCs produced in dual confrontation systems performed in two culture media by strains ( IMI206040, T1 and T3, and sp. T2) on . We analysed the VOC profiles using gas chromatography coupled with mass spectrometry. The Luria Bertani (LB) medium stimulated the production of VOCs with antifungal properties in most systems. We identified 2-pentyl furan, dimethyl disulfide, and α-phellandrene and determined their antifungal activity in vitro. The equimolar mixture of those VOCs (250 µM ea.) resulted in 14% diametral growth inhibition. The infective ability and disease severity caused by the mycelia exposed to the VOCs mixture were notably diminished in strawberry leaves. Application of these VOCs as biofumigants may contribute to the management of anthracnose. LB represents a feasible strategy for identifying novel VOCs produced by strains with antifungal properties.
管理由植物病原真菌引起的植物病害，如由 种引起的炭疽病，具有挑战性。人们已采用不同方法来鉴定具有抗生素特性的化合物。 菌株是具有抗真菌特性的新型分子的来源，包括挥发性有机化合物（VOCs），其产生受培养基营养成分的影响。在本研究中，我们评估了 菌株（IMI206040、 T1和T3以及 sp. T2）在两种培养基中进行的双对抗系统中产生的VOCs对 的影响。我们使用气相色谱-质谱联用分析了VOCs谱图。在大多数系统中，Luria Bertani（LB）培养基刺激了具有抗真菌特性的VOCs的产生。我们鉴定出2-戊基呋喃、二甲基二硫和α-水芹烯，并测定了它们在体外的抗真菌活性。这些VOCs的等摩尔混合物（各250 µM）导致直径生长抑制14%。暴露于VOCs混合物的菌丝体引起的感染能力和病害严重程度在草莓叶片中显著降低。将这些VOCs用作生物熏蒸剂可能有助于炭疽病的管理。LB是鉴定具有抗真菌特性的 菌株产生的新型VOCs的可行策略。
