Minardi Carlotta, Bersanetti Davide, Sarlin Essi, Santala Ville, Mangayil Rahul
Faculty of Engineering and Natural Sciences, Tampere University, 33100 Tampere, Finland.
Department of Chemical Engineering, Chemical and Biochemical Reactor Engineering and Safety (CREaS @ De Nayer), KU Leuven, J. De Nayerlaan 5, 2560 Sint-Katelijne-Waver, Belgium.
Microorganisms. 2024 Oct 20;12(10):2095. doi: 10.3390/microorganisms12102095.
Bacterial nanocellulose (BC) has attracted significant attention across a wide array of applications due to its distinctive characteristics. Recently, there has been increasing interest in leveraging waste biomass to improve sustainability in BC biogenesis processes. This study focuses on optimizing the citrus pulp waste (CPW) medium to enhance BC production using . The screening of initial medium pH, yeast extract, CPW sugar and inoculum concentrations was conducted using the Plackett-Burman design, with BC yield (mgDW/gCPW) as the model response. The significant parameters, i.e., CPW sugars and yeast extract concentrations, were optimized using response surface methodology, employing a five-level, two-factor central composite design. The optimized CPW-based growth medium resulted in a final yield of 66.7 ± 5.1 mgDW/gCPW, representing a 14-fold increase compared to non-optimized conditions (4.3 ± 0.4 mgBC/gCPW). Material characterization analysis indicated that the produced BC showed high thermal stability (30% mass retained at 600 °C) and a crystallinity index value of 71%. Additionally, to enhance process sustainability, spent baker's yeast hydrolysate (BYH) was assessed as a substitute for yeast extract, leading to a final BC titer of 9.3 ± 0.6 g/L.
细菌纳米纤维素（BC）因其独特的特性在广泛的应用中引起了广泛关注。最近，利用废弃生物质来提高BC生物合成过程的可持续性受到越来越多的关注。本研究重点优化柑橘果肉废料（CPW）培养基，以提高使用……生产BC的产量。使用Plackett-Burman设计对初始培养基pH值、酵母提取物、CPW糖和接种物浓度进行筛选，以BC产量（mgDW/gCPW）作为模型响应。使用响应面法，采用五级、两因素中心复合设计对显著参数，即CPW糖和酵母提取物浓度进行优化。优化后的基于CPW的生长培养基最终产量为66.7±5.1 mgDW/gCPW，与未优化条件（4.3±0.4 mgBC/gCPW）相比提高了14倍。材料表征分析表明，所生产的BC具有高热稳定性（在600℃下保留30%的质量）和71%的结晶度指数值。此外，为了提高过程的可持续性，评估了废弃面包酵母水解物（BYH）作为酵母提取物的替代品，最终BC滴度为9.3±0.6 g/L。
