Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10 Str., 10-748 Olsztyn, Poland.
Nutrients. 2024 Oct 10;16(20):3430. doi: 10.3390/nu16203430.
BACKGROUND/OBJECTIVES: Cow's milk allergy is one of the most common food allergies in children, and its pathomechanism is still under investigation. Recently, an increasing number of studies have linked food allergy to intestinal barrier dysfunction. The present study aimed to investigate changes in the intestinal microenvironment during the development of -lactoglobulin (-lg) allergy under conditions of early intestinal dysfunction.
BALB/c mice received intraperitoneal -lg with Freund's adjuvant, followed by oral -lg while receiving dextran sulphate sodium salt (DSS) in their drinking water (0.2% /). The immunized group without DSS and the groups receiving saline, oral -lg, or DSS served as controls.
The study showed that the immunization effect was greater in mice with mild intestinal barrier dysfunction. Although DSS did not affect the mice's humoral response to -lg, in combination with -lg, it significantly altered their cellular response, affecting the induction and distribution of T cells in the inductive and peripheral tissues and the activation of immune mediators. Administration of -lg to sensitized mice receiving DSS increased disease activity index (DAI) scores and pro-inflammatory cytokine activity, altered the distribution of claudins and zonulin 1 (ZO-1) in the colonic tissue, and negatively affected the balance and activity of the gut microbiota.
The research model used appears attractive for studying food allergen sensitization, particularly in relation to the initial events leading to mucosal inflammation and the development of food hypersensitivity.
背景/目的：牛奶过敏是儿童最常见的食物过敏之一，其发病机制仍在研究中。最近，越来越多的研究将食物过敏与肠道屏障功能障碍联系起来。本研究旨在探讨早期肠道功能障碍条件下β-乳球蛋白（β-lg）过敏发展过程中肠道微环境的变化。
BALB/c 小鼠接受腹腔内β-lg 佐剂，然后在饮用水中给予葡聚糖硫酸钠（DSS）（0.2%/）的同时口服β-lg。无 DSS 的免疫组和接受生理盐水、口服β-lg 或 DSS 的组作为对照组。
研究表明，在肠道屏障功能轻度障碍的小鼠中，免疫效果更大。尽管 DSS 不影响小鼠对β-lg 的体液反应，但与β-lg 结合时，它显著改变了它们的细胞反应，影响了诱导和外周组织中 T 细胞的诱导和分布，以及免疫介质的激活。给予 DSS 致敏的小鼠β-lg 可增加疾病活动指数（DAI）评分和促炎细胞因子活性，改变结肠组织中闭合蛋白和 zonulin 1（ZO-1）的分布，并对肠道微生物群的平衡和活性产生负面影响。
所使用的研究模型似乎对研究食物过敏原致敏很有吸引力，尤其是在研究导致粘膜炎症和食物过敏发展的初始事件方面。
