Morocho Vladimir, Aguilar Yolanda, Cruz Claudia, Cumbicus Nixon, Andrade Jose Miguel, Montalvan Mayra
Departamento de Química, Universidad Técnica Particular de Loja, Loja 110150, Ecuador.
Departamento de Ciencias Biológicas y Agropecuarias, Universidad Técnica Particular de Loja (UTPL), San Cayetano s/n, Loja 1101608, Ecuador.
Plants (Basel). 2024 Oct 10;13(20):2834. doi: 10.3390/plants13202834.
The essential oil was obtained by steam distillation, using a Clevenger apparatus, from the pericarp of the fruit of from Ecuador. The qualitative and quantitative analyses were performed by gas chromatography coupled with mass spectrometry (GC-MS) and flame ionization detection (GC-FID) on two capillary columns with non-polar DB-5ms and a polar HP-INNOWax stationary phase. Thirty-three components were identified, accounting for 99.62% and 99.30% total essential oil. The essential oil was dominated by oxygenated monoterpenes (90.21-89.21%), respectively. The main constituents of the essential oil were α-thujone (70.26-70.38%), β-thujone (10.78-10.90%), terpinen-4-ol (4.15-4.06%), and sabinene (3.60-4.02%). Enantioselective analysis by GC was realized on a β-cyclodextrin-based chiral column (2,3-diethyl-6-tert-butyldimethylsilyl-β-cyclodextrin) in this analysis, determining three couples of enantiomers, which exhibited the compound (,,)-(+)-α-thujone with an enantiomeric excess of 84.40%.
采用克利夫兰装置通过水蒸气蒸馏法从厄瓜多尔水果的果皮中提取精油。在两根分别带有非极性DB - 5ms和极性HP - INNOWax固定相的毛细管柱上，通过气相色谱 - 质谱联用（GC - MS）和火焰离子化检测（GC - FID）进行定性和定量分析。共鉴定出33种成分，分别占精油总量的99.62%和99.30%。精油分别以氧化单萜类化合物为主（90.21 - 89.21%）。精油的主要成分是α - 侧柏酮（70.26 - 70.38%）、β - 侧柏酮（10.78 - 10.90%）、萜品烯 - 4 - 醇（4.15 - 4.06%）和桧烯（3.60 - 4.02%）。在本次分析中，通过基于β - 环糊精的手性柱（2,3 - 二乙基 - 6 - 叔丁基二甲基硅烷基 - β - 环糊精）对映体选择性分析实现了对三对对映体的测定，其中化合物（,,） - （ + ） - α - 侧柏酮的对映体过量为84.40%。
