Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science , Tohoku University , Sendai , Miyagi 980-0845 , Japan.
School of Food, Agricultural and Environmental Sciences , Miyagi University , Sendai , Miyagi 982-0215 , Japan.
J Agric Food Chem. 2018 Oct 31;66(43):11320-11329. doi: 10.1021/acs.jafc.8b03273. Epub 2018 Oct 19.
Luteolin is a flavonoid present in plants in the form of aglycone or glucosides. In this study, luteolin glucosides (i.e., luteolin-7- O-β-d-glucoside, luteolin-7- O-[2-(β-d-apiosyl)-β-d-glucoside], and luteolin-7- O-[2-(β-d-apiosyl)-6-malonyl-β-d-glucoside]) prepared from green pepper leaves as well as luteolin aglycone were orally administered to rats. Regardless of the administered luteolin form, luteolin glucuronides were mainly detected from plasma and organs. Subsequently, luteolin aglycone, the most absorbed form of luteolin in rats, was orally administered to humans. As a result, luteolin-3'- O-sulfate was mainly identified from plasma, suggesting that not only luteolin form but also animal species affect the absorption and metabolism of luteolin. When LPS-treated RAW264.7 cells were treated with luteolin glucuronides and luteolin sulfate (the characteristic metabolites identified from rats and humans, respectively), the different luteolin conjugates were metabolized in different ways, suggesting that such difference in metabolism results in their difference in anti-inflammatory effects.
木樨草素是一种类黄酮，以游离形式或糖苷形式存在于植物中。在这项研究中，从青椒叶片中提取的木樨草素糖苷（即木樨草素-7-O-β-D-葡萄糖苷、木樨草素-7-O-[2-（β-D-吡喃葡萄糖基）-β-D-葡萄糖苷]和木樨草素-7-O-[2-（β-D-吡喃葡萄糖基）-6- 马来酰基-β-D-葡萄糖苷]）以及木樨草素游离形式被口服给予大鼠。无论给予的木樨草素形式如何，木樨草素葡萄糖醛酸苷主要从血浆和器官中检测到。随后，作为大鼠中木樨草素最易吸收的形式，木樨草素游离形式被口服给予人类。结果，主要从血浆中鉴定出木樨草素-3'-O-硫酸盐，表明不仅木樨草素的形式，而且动物种类也会影响木樨草素的吸收和代谢。当用 LPS 处理 RAW264.7 细胞时，用木樨草素葡萄糖醛酸苷和木樨草素硫酸盐（分别是从大鼠和人类中鉴定出的特征代谢物）进行处理，不同的木樨草素缀合物以不同的方式进行代谢，表明这种代谢差异导致其抗炎作用的差异。
