Hirose Shogo, Sakai Kaito, Kobayashi Sawa, Tsuro Masato, Morikami Atsushi, Tsukagoshi Hironaka
Faculty of Agriculture, Meijo University.
Plant Biotechnol (Tokyo). 2024 Jun 25;41(2):111-120. doi: 10.5511/plantbiotechnology.24.0124a.
Aromatic compounds play essential roles in plant physiology and various industries because of their unique fragrances and beneficial properties. In this study, we investigated the transport and biosynthesis of eugenol, a prominent aromatic compound, within the genus, using grafting experiments. Grafting sweet basil () scions onto diverse rootstocks, including tobacco () and thyme (), revealed that eugenol is transported from the shoot to the root across distinct plant species. Furthermore, grafting within the genus, which includes , , and , resulted in variations in eugenol transport and accumulation. The eugenol content in the shoots remained constant across all combinations, whereas the root eugenol levels varied depending on the scion-rootstock pair. To elucidate the biosynthetic capabilities of eugenol in roots, we performed in vitro enzyme assays using crude protein extracts from roots, which revealed that eugenol can be synthesized in roots in addition to being transported. Expression analysis of eugenol synthase (EGSs) genes showed that expression was influenced by grafting in roots, suggesting compensation by other EGSs. Our results suggest that eugenol transport and biosynthesis are multifaceted processes influenced by the interactions between different species and tissues. The potential to engineer eugenol levels in rootstocks lacking biosynthetic capacity has potential applications in agriculture and industry. This study reveals the dynamic interplay between eugenol transport and biosynthesis in the genus, providing insights into the manipulation of aromatic compound production in plants.
芳香化合物因其独特的香气和有益特性，在植物生理学和各个行业中发挥着重要作用。在本研究中，我们利用嫁接实验，研究了丁香酚（一种重要的芳香化合物）在该属植物中的运输和生物合成。将甜罗勒（）接穗嫁接到包括烟草（）和百里香（）在内的不同砧木上，结果表明丁香酚能在不同植物物种间从地上部分运输到根部。此外，在包括、和的该属植物内进行嫁接，导致丁香酚的运输和积累出现差异。所有组合中地上部分的丁香酚含量保持恒定，而根部丁香酚水平则因接穗 - 砧木组合而异。为了阐明丁香酚在根部的生物合成能力，我们使用从根部提取的粗蛋白提取物进行了体外酶活性测定，结果表明丁香酚除了能被运输外，还能在根部合成。丁香酚合酶（EGSs）基因的表达分析表明，在根部的表达受嫁接影响，这表明可能有其他EGSs进行了补偿。我们的结果表明，丁香酚的运输和生物合成是受不同物种和组织间相互作用影响的多方面过程。在缺乏生物合成能力的砧木中调控丁香酚水平的潜力在农业和工业中具有潜在应用价值。本研究揭示了该属植物中丁香酚运输和生物合成之间的动态相互作用，为操纵植物中芳香化合物的生产提供了见解。
