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使用香蕉富集培养基提高M. W. 伍德与P. 泰勒以及J. J. 史密斯培养物的生长速率

Improvement of Growth Rate in Culture of M. W. Wood & P. Taylor and J. J. Smith using Banana Enrichment Media.

作者信息

Yalapuspita Dyah Carinae, Handini Elizabeth, Aprilianti Popi, Isnaini Yupi, Semiarti Endang

机构信息

Graduate School, Universitas Gadjah Mada, Jl. Teknika Utara, Sleman 55281, Yogyakarta, Indonesia.

Research Center for Plant Conservation and Botanic Gardens, National Research and Innovation Agency, Techno Park of Dr. (H.C.) Ir. Soekarno, Jl. Raya Jakarta - Bogor KM 46, Cibinong, Bogor 16911, West Java, Indonesia.

出版信息

Trop Life Sci Res. 2024 Oct;35(3):109-120. doi: 10.21315/tlsr2024.35.3.5. Epub 2024 Oct 7.

DOI:10.21315/tlsr2024.35.3.5
PMID:39464666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11507972/
Abstract

and have unique labellum colour and shaped like lady's slippers. These orchids are from the Cochlopetalum section, which is exclusively found in Sumatra and Java. There are so many people that desire to collect these plants illegally. Due to extensive commercial exploitation, is in danger of going extinct. Tissue culture techniques are utilised to conserve threatened orchid germplasm in a short time. The success of the culture depends on the accuracy of the basic media composition used. The Ambon Lumut banana (ALB) can accelerate plant growth and cell division. Banana added to the culture medium was prepared by mashing the ripe flesh (3.5 months old) using a mortar. This research aims to investigate the effect of banana homogenate supplemented media for the orchids and based on the parameters of difference of plant height (calculated from the base of the stem to the tip of the plant stem), number of leaves, and number of roots. The measurement method was carried out using a ruler with a centimetre scale. Observations and documentation were carried out once a week for 7 weeks after planting (WAP) for and . The results showed that ½ Murashige and Skoog (MS) + ALB homogenate is a better medium for and growth than media without banana homogenate. The highest values of plant height, leaf growth and root growth of with banana homogenate were 0.44 cm, 0.63 leaves, and 0.50 roots, respectively. The highest values of plant height and leaf growth of were 0.75 cm and 1.90 leaves, respectively. Culture medium added banana homogenate was able to support the propagation of plants, some of which are returned to nature and others used for industrial purposes (conventionally cultivated by the community).

摘要

并且具有独特的唇瓣颜色,形状像拖鞋。这些兰花属于Cochlopetalum组,仅在苏门答腊和爪哇发现。有很多人渴望非法收集这些植物。由于广泛的商业开发,其面临灭绝的危险。组织培养技术被用于在短时间内保护受威胁的兰花种质。培养的成功取决于所用基本培养基成分的准确性。安汶卢穆特香蕉(ALB)可以促进植物生长和细胞分裂。添加到培养基中的香蕉是通过用研钵将成熟果肉(3.5个月大)捣碎制备的。本研究旨在基于株高差异(从茎基部到植物茎尖计算)、叶片数量和根系数量等参数,研究添加香蕉匀浆的培养基对[两种兰花名称未给出]的影响。测量方法是使用带有厘米刻度的尺子进行。种植后(种植周数,WAP),对[两种兰花名称未给出]每周进行一次观察和记录,持续7周。结果表明,对于[两种兰花名称未给出]的生长,1/2的Murashige和Skoog(MS)培养基 + ALB匀浆比不添加香蕉匀浆的培养基更好。添加香蕉匀浆时,[一种兰花名称未给出]的株高、叶片生长和根系生长的最高值分别为0.44厘米、0.63片叶和0.50条根。[另一种兰花名称未给出]的株高和叶片生长的最高值分别为0.75厘米和1.90片叶。添加香蕉匀浆的培养基能够支持植物繁殖,其中一些回归自然,另一些用于工业用途(由社区传统种植)。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/90eda4144ec3/TLSR_35-3-109-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/8eb9bc815960/TLSR_35-3-109-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/3974101273ae/TLSR_35-3-109-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/90eda4144ec3/TLSR_35-3-109-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/8eb9bc815960/TLSR_35-3-109-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/3974101273ae/TLSR_35-3-109-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c91/11507972/90eda4144ec3/TLSR_35-3-109-g003.jpg

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本文引用的文献

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PLoS One. 2021 Jul 8;16(7):e0253366. doi: 10.1371/journal.pone.0253366. eCollection 2021.
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Ovule Development and Transformation of Maudiae by -Mediated Ovary-Injection.胚珠发育与 Maudiae 的转变——介导的子房注射。
Int J Mol Sci. 2020 Dec 23;22(1):84. doi: 10.3390/ijms22010084.
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The importance of thiamine (vitamin B) in plant health: From crop yield to biofortification.
硫胺素(维生素 B)在植物健康中的重要性:从作物产量到生物强化。
J Biol Chem. 2020 Aug 21;295(34):12002-12013. doi: 10.1074/jbc.REV120.010918. Epub 2020 Jun 17.