Mariya Sela S, Saepuloh Uus, Febriani Novi, Perwitasari-Farajallah Dyah, Iskandriati Diah, Darusman Huda S, Pamungkas Joko
Primate Research Center, IPB University, Jl Lodaya 2 No 5 Bogor, West Java Indonesia.
Center for Biomedical Research, National Research, and Innovation Agency of Indonesia, Cibinong-Bogor, West Java, Indonesia.
Trop Life Sci Res. 2024 Oct;35(3):293-305. doi: 10.21315/tlsr2024.35.3.13. Epub 2024 Oct 7.
The pigtailed monkey () is one of the species that have potency like the cynomolgus monkey that is widely used as an animal model for asthma study. The gene has potential as a genetic marker because of the secreted chemokine that plays a role in asthma. The aims of this research are to characterise the gene of pigtailed monkey, compare the structure of their gene with other primate species and determine model 3D structure protein prediction of CCL7 protein. The amplicons were sequenced, and the results were analysed by the bioinformatics technique. The 3D CCL7 protein structure was predicted using I-TASSER. We have isolated 2221 bp sequences gene and 109 amino acids from pigtailed monkey. Variation of gene sequence between pigtailed monkey and other primate species ( and ) was found in exon 1, exon 2 and exon 3 as CDS (Coding DNA Sequence) region. The analysis homology of nucleotides and amino acid sequences of the gene indicated that the pigtailed monkey and three other primate species have a high homology rate with an identity score above 90%. Meanwhile, a comparative analysis of CDS and amino acid regions showed that the pigtailed monkey also has the highest similarity with the three other primate species with more than 90% identity score. The 3D structure protein prediction model of the CCL7 pigtailed monkey revealed the highest similarity with with an identity value of about 95%. Therefore, the pigtailed monkey gene has high similarity with , which means that based on molecular similarity, the pigtailed monkey has the potential to be an animal model for asthma study, especially the study of molecular and the role of CCL7 in asthma pathogenesis.
豚尾猴（）是具有与食蟹猴相似潜力的物种之一，食蟹猴被广泛用作哮喘研究的动物模型。该基因具有作为遗传标记的潜力，因为其分泌的趋化因子在哮喘中起作用。本研究的目的是对豚尾猴的基因进行表征，将其基因结构与其他灵长类物种进行比较，并确定CCL7蛋白的3D结构蛋白预测模型。对扩增子进行测序，并采用生物信息学技术分析结果。使用I-TASSER预测CCL7蛋白的3D结构。我们从豚尾猴中分离出了2221 bp的基因序列和109个氨基酸。在作为编码DNA序列（CDS）区域的外显子1、外显子2和外显子3中发现了豚尾猴与其他灵长类物种（和）之间的基因序列变异。对该基因的核苷酸和氨基酸序列的同源性分析表明，豚尾猴与其他三种灵长类物种具有较高的同源率，同一性得分超过90%。同时，对CDS和氨基酸区域的比较分析表明，豚尾猴与其他三种灵长类物种也具有最高的相似性，同一性得分超过90%。豚尾猴CCL7的3D结构蛋白预测模型显示与的相似性最高，同一性值约为95%。因此，豚尾猴基因与具有高度相似性，这意味着基于分子相似性，豚尾猴有潜力成为哮喘研究的动物模型，特别是分子研究以及CCL7在哮喘发病机制中的作用研究。
