Graumlich T R, Stevenson K E
Appl Environ Microbiol. 1979 Sep;38(3):461-5. doi: 10.1128/aem.38.3.461-465.1979.
Resting cells of Saccharomyces cerevisiae Y25 were heated at 56 degrees C for 0 to 2 min. Respiratory activity of the cells reflected the severity of the heat stress. The endogenous respiration was approximately 50 microliter of O2/mg per h for cells heated for 2 min at 56 degrees C as compared with 2 microliter of O2/mg per h for nonheated cells. There was a distinct decrease in respiration after 1 to 3 h, and after 20 h the respiration rate of heated cells was less than that of nonheated cells. Along with increased rates of endogenous respiration, respiratory quotients of cells were altered after heat stress. Addition of 2,4-dinitrophenol stimulated O2 (uptake) in nonheated cells but decreased O2 (uptake) of heated cells. Due to the high rate of endogenous respiration, addition of glucose resulted in no substantial change in the rate of respiration of heated cells. However, addition of glucose prolonged the presence of the high rates of respiration observed in heated cells.
将酿酒酵母Y25的静止细胞在56℃加热0至2分钟。细胞的呼吸活性反映了热应激的严重程度。与未加热细胞每毫克每小时约2微升氧气的内源性呼吸相比，在56℃加热2分钟的细胞内源性呼吸约为每毫克每小时50微升氧气。1至3小时后呼吸明显下降，20小时后加热细胞的呼吸速率低于未加热细胞。随着内源性呼吸速率的增加，热应激后细胞的呼吸商发生改变。添加2,4-二硝基苯酚刺激未加热细胞的氧气摄取，但降低加热细胞的氧气摄取。由于内源性呼吸速率高，添加葡萄糖不会使加热细胞的呼吸速率发生实质性变化。然而，添加葡萄糖延长了加热细胞中观察到的高呼吸速率的持续时间。
