Krzyżek Paweł, Migdał Paweł, Tusiewicz Kaja, Zawadzki Marcin, Szpot Paweł
Department of Microbiology, Faculty of Medicine, Wroclaw Medical University, Wroclaw, Poland.
Department of Bees Breeding, Institute of Animal Husbandry, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
Front Pharmacol. 2024 Oct 14;15:1477317. doi: 10.3389/fphar.2024.1477317. eCollection 2024.
causes chronic gastric diseases in nearly 50% of people around the world. It is suggested that biofilm formation has a pronounced effect on the dynamic resistance spread and recurrence of these infections.
To mimic the scenario of therapeutic ineffectiveness, we investigated the impact of sub-minimal inhibitory concentrations (sub-MICs) of antibiotics on the development and parameters of biofilms produced by clinical strains.
We observed that constant exposure of planktonic forms to metronidazole or levofloxacin stimulated the speed of autoaggregation and the amount of extracellular matrix, resulting in increased dimensions of the developed biofilms. Contrary to this, continuous exposure to clarithromycin negatively affected a number of biofilm-related reactions and led to the biofilm-weakening effect. Through assessing the membrane fatty acid profiles of antibiotic-exposed cells, we confirmed that metronidazole and levofloxacin induced a biofilm-like phenotype, while clarithromycin kept bacteria in a planktonic form.
Our results suggest that sub-MICs of antibiotics affect the biochemical and biophysical properties of the developing biofilm of strains and may impact the effectiveness of antibiotic treatment.
幽门螺杆菌在全球近50%的人群中引发慢性胃部疾病。有研究表明，生物膜的形成对这些感染的动态耐药性传播及复发有着显著影响。
为模拟治疗无效的情况，我们研究了抗生素的亚最小抑菌浓度（sub-MICs）对临床菌株所产生生物膜的形成及参数的影响。
我们观察到，浮游形式的细菌持续暴露于甲硝唑或左氧氟沙星会刺激自聚集速度及细胞外基质的量，从而导致所形成生物膜的尺寸增大。与此相反，持续暴露于克拉霉素会对一些与生物膜相关的反应产生负面影响，并导致生物膜弱化效应。通过评估暴露于抗生素的细胞的膜脂肪酸谱，我们证实甲硝唑和左氧氟沙星诱导了生物膜样表型，而克拉霉素使细菌保持浮游形式。
我们的结果表明，抗生素的亚最小抑菌浓度会影响幽门螺杆菌生物膜形成过程中的生化和生物物理特性，并可能影响抗生素治疗的效果。
