Department of Biological Sciences and Biotechnology, Hannam University Jeonmin-dong, Yuseong-gu, Daejeon 34430, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006566.
A facultative anaerobic Gram-negative bacterium, designated as strain 2205BS29-5, was isolated from a marine sponge, , in Beomseom on Jeju Island, Republic of Korea, and taxonomically characterized. The cells were catalase and oxidase positive, non-motile, coccoid-rod shaped and capable of poly-β-hydroxybutyrate production. Growth was observed at 10-37 °C (optimum, 25 °C) and pH 5.0-8.0 (optimum, pH 7.0), and in the presence of 0-9% NaCl (w/v) (optimum, 3.0-4.0%). The major cellular fatty acids and respiratory quinone were identified as summed feature 8 (C 7/C 6) and Q-10, respectively. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, four phosphoglycolipids, two unidentified amino lipids and eight unidentified lipids. The DNA G+C content was 67.8%. Strain 2205BS29-5 was most closely represented by 11-3 and MJ17 with 97.8 and 97.5% 16S rRNA gene sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA genes and whole-genome sequences showed that strain 2205BS29-5 was affiliated with the genus . Genomic analysis showed that strain 2205BS29-5 could synthesize vitamin B family (folate and cobalamin) and ectoine. The average nucleotide identity and digital DNA-DNA hybridization values between strain 2205BS29-5T and 11-3 were 77.1% and 18.8%, respectively, and with MJ17 were 78.4 and 21.2%, respectively. Based on phylogenetic, chemotaxonomic and genome relatedness analyses, strain 2205BS29-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2205BS29-5 (=LMG 33062 =KACC 23240).
一种兼性厌氧革兰氏阴性细菌，被命名为 2205BS29-5 株，从韩国济州岛的 Beomseom 的海洋海绵中分离出来，并进行了分类学特征描述。该细胞呈过氧化氢酶和氧化酶阳性，非运动性，球形-杆状，能够产生聚-β-羟基丁酸。在 10-37°C（最佳温度为 25°C）和 pH 5.0-8.0（最佳 pH 为 7.0）的条件下，以及在 0-9%NaCl（w/v）（最佳 3.0-4.0%）的条件下可以生长。主要的细胞脂肪酸和呼吸醌分别为特征 8（C 7/C 6）和 Q-10。主要的极性脂包括二磷脂酰甘油、磷脂酰甘油、四种磷酸甘油酯、两种未鉴定的氨基酸脂和八种未鉴定的脂。DNA G+C 含量为 67.8%。2205BS29-5 株与 11-3 和 MJ17 的 16S rRNA 基因序列相似性最高，分别为 97.8%和 97.5%。基于 16S rRNA 基因和全基因组序列的系统发育分析表明，2205BS29-5 株属于 属。基因组分析表明，2205BS29-5 株可以合成维生素 B 家族（叶酸和钴胺素）和海藻糖。与 11-3 株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 77.1%和 18.8%，与 MJ17 株的分别为 78.4%和 21.2%。基于系统发育、化学分类和基因组相关性分析，2205BS29-5 株代表了一个新的属种，建议命名为 sp. nov.。模式菌株为 2205BS29-5（=LMG 33062 =KACC 23240）。
