Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, PR China.
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):1132-1137. doi: 10.1099/ijs.0.044180-0. Epub 2012 Jun 29.
A facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-reaction-negative, coccoid to short rod-shaped strain, designated FLN-7(T), was isolated from activated sludge of a wastewater biotreatment facility. The strain was able to hydrolyse amide pesticides (e.g. diflubenzuron, propanil, chlorpropham and dimethoate) through amide bond cleavage. Strain FLN-7(T) grew at 4-42 °C (optimum 28 °C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-5.0 % (w/v) NaCl (optimum 1.0 %). The major respiratory quinone was ubiquinone-10. The major cellular fatty acid was C18 : 1ω7c. The genomic DNA G+C content of strain FLN-7(T) was 66.4±0.5 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain FLN-7(T) was a member of the genus Paracoccus and showed highest 16S rRNA gene sequence similarities with Paracoccus aminovorans JCM 7685(T) (99.2 %), P. denitrificans DSM 413(T) (97.8 %), P. yeei CDC G1212(T) (97.3 %) and P. thiocyanatus THI 011(T) (97.1 %). Strain FLN-7(T) showed low DNA-DNA relatedness with P. aminovorans KACC 12261(T ()36.5±3.4 %), P. denitrificans KACC 12251(T) (30.5±2.6 %), P. yeei CCUG 46822(T) (26.2±2.4 %) and P. thiocyanatus KACC 13901(T) (15.5±0.9 %). Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition and biochemical characteristics, strain FLN-7(T) was clearly distinguished from all recognized species of the genus Paracoccus and should be classified in a novel species, for which the name Paracoccus huijuniae sp. nov. is proposed. The type strain is FLN-7(T) ( = KACC 16242(T) = ACCC 05690(T)).
一株兼性厌氧、不产芽孢、不运动、过氧化氢酶和氧化酶阳性、革兰氏反应阴性、呈球形到短杆状的菌株，被命名为 FLN-7(T)，从废水生物处理设施的活性污泥中分离得到。该菌株能够通过酰胺键裂解水解酰胺类农药（如除虫脲、丙草胺、氯丙胺和乐果）。FLN-7(T) 菌株在 4-42°C（最佳 28°C）、pH5.0-8.0（最佳 pH7.0）和 0-5.0%（w/v）NaCl（最佳 1.0%）下生长。主要呼吸醌为泛醌-10。主要细胞脂肪酸为 C18:1ω7c。FLN-7(T) 菌株的基因组 DNA G+C 含量为 66.4±0.5mol%。主要极性脂为磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰胆碱和一种未鉴定的糖脂。基于 16S rRNA 基因序列的系统发育分析表明，菌株 FLN-7(T) 是 Paracoccus 属的一个成员，与 Paracoccus aminovorans JCM 7685(T)（99.2%）、P. denitrificans DSM 413(T)（97.8%）、P. yeei CDC G1212(T)（97.3%）和 P. thiocyanatus THI 011(T)（97.1%）的 16S rRNA 基因序列相似度最高。FLN-7(T) 菌株与 P. aminovorans KACC 12261(T)（36.5±3.4%）、P. denitrificans KACC 12251(T)（30.5±2.6%）、P. yeei CCUG 46822(T)（26.2±2.4%）和 P. thiocyanatus KACC 13901(T)（15.5±0.9%）的 DNA-DNA 相关性较低。基于系统发育分析、DNA-DNA 杂交、全细胞脂肪酸组成和生化特性，FLN-7(T) 菌株与 Paracoccus 属所有已识别种明显不同，应归类为一个新种，命名为 Paracoccus huijuniae sp. nov.。模式菌株为 FLN-7(T)（=KACC 16242(T)=ACCC 05690(T)）。
