Department of Biochemistry, Alex Ekwueme Federal University, Ndufu-Alike, Ikwo, Nigeria.
Department of Biochemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.
Curr Microbiol. 2024 Oct 31;81(12):436. doi: 10.1007/s00284-024-03966-5.
Although an environmental contaminant, Alcaligenes sp. are now increasingly reported in clinical infections. Here, we present a whole-genome sequence of an extended spectrum beta-lactamase (ESBL) Alcaligenes nematophilus strain EBU-23 encoding beta-lactamase, bla gene, isolated from the stool of a hospitalized infant with acute gastroenteritis in a tertiary hospital in Nigeria. Antibiotics susceptibility test revealed that the isolate was resistant to the β-lactams, cephalosporins, and penicillin, β-lactam combination, and the fluoroquinolones. Genomic analysis exposed the presence of a class A beta-lactam hydrolase gene and multiple multidrug efflux permease which may be responsible for the beta-lactamase and multidrug resistance observed with the isolate. To the best our knowledge, we describe the first whole-genome sequence of a multidrug-resistant A. nematophilus implicated in acute childhood gastroenteritis in Nigeria.
虽然 Alcaligenes sp. 是一种环境污染物，但现在越来越多的临床感染报告中都出现了它。在这里，我们展示了一株从尼日利亚一家三级医院住院的急性肠胃炎婴儿粪便中分离出的产超广谱β-内酰胺酶（ESBL）的食酸寡养单胞菌（Alcaligenes nematophilus）菌株 EBU-23 的全基因组序列。抗生素敏感性测试显示，该分离株对β-内酰胺类、头孢菌素类和青霉素类、β-内酰胺类联合药物以及氟喹诺酮类药物具有耐药性。基因组分析揭示了存在一种 A 类β-内酰胺水解酶基因和多种多药外排渗透酶，这可能是导致该分离株出现β-内酰胺酶和多药耐药的原因。据我们所知，我们描述了尼日利亚首例与儿童急性肠胃炎相关的多药耐药食酸寡养单胞菌的全基因组序列。
