Sahatsanon Katatikarn, Sivapirunthep Panneepa, Sringarm Korawan, Arjin Chaiwat, Hnokaew Patipan, Chaweewan Kamon, Chaosap Chanporn
Doctoral Program in Innovative Tropical Agriculture, Department of Agricultural Education, School of Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Department of Agricultural Education, School of Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Anim Biosci. 2025 Apr;38(4):717-727. doi: 10.5713/ab.24.0556. Epub 2024 Oct 28.
This study aimed to assess the impact of host-specific and locally isolated multistrain probiotics on piglet performance, mortality, inflammatory responses, and gut microbiome.
A total of 52 piglet litters-34 from Landrace sows and 18 from Large White sows-were allocated to two groups: a control group and a multi-strain probiotic group. The probiotic group comprised seven strains of lactic acid bacteria (MLAB): Lactobacillus brevis, Lactobacillus reuteri, Lactobacillus paraplantarum, Lactococcus lactis, Lactobacillus pentosus, Weissella cibaria, and Pediococcus pentosaceus. Each strain was included in equal concentrations, resulting in a final liquid mixture containing 109 colony forming units/mL. The MLAB group received the probiotics orally starting from 7 days of age until weaning at four weeks. Following weaning, supplementation continued via feed spraying for an additional four weeks.
MLAB supplementation did not significantly affect piglet performance but showed a trend towards reducing the mortality rate (p = 0.06). It influenced the inflammatory response by upregulating the expression of anti-inflammatory cytokines interleukin (IL)-4 and IL-10 (p<0.05). Microbial community analysis indicated that MLAB supplementation increased both microbial diversity (Simpson index: p = 0.06) and species richness (Chao1 index: p = 0.02). Piglets receiving MLAB had a significantly higher abundance of the phylum Firmicutes (p<0.01) compared to the control group, while the abundance of the phylum Bacteroidota was markedly reduced (p<0.01). In addition, the relative abundance of the bacterial genera Prevotellaceae_NK3B31 (p<0.01) and Chlamydia (p = 0.03) was lower in the MLAB group.
Overall, these results suggest that while MLAB supplementation does not directly improve piglet growth performance, it has the potential to improve immune function and promote a healthier gut microbiota in weaning piglets, which could ultimately reduce mortality rates.
本研究旨在评估宿主特异性和本地分离的多菌株益生菌对仔猪生长性能、死亡率、炎症反应和肠道微生物群的影响。
总共52窝仔猪——34窝来自长白母猪，18窝来自大白母猪——被分为两组：对照组和多菌株益生菌组。益生菌组包含7株乳酸菌（MLAB）：短乳杆菌、罗伊氏乳杆菌、副植物乳杆菌、乳酸乳球菌、戊糖乳杆菌、西巴乳杆菌和戊糖片球菌。每种菌株的浓度相等，最终得到一种含有109个菌落形成单位/毫升的液体混合物。MLAB组从7日龄开始口服益生菌，直至四周龄断奶。断奶后，通过饲料喷洒继续补充四周。
补充MLAB对仔猪生长性能没有显著影响，但有降低死亡率的趋势（p = 0.06）。它通过上调抗炎细胞因子白细胞介素（IL）-4和IL-10的表达来影响炎症反应（p<0.05）。微生物群落分析表明，补充MLAB增加了微生物多样性（辛普森指数：p = 0.06）和物种丰富度（Chao1指数：p = 0.02）。与对照组相比，接受MLAB的仔猪厚壁菌门的丰度显著更高（p<0.01），而拟杆菌门的丰度则显著降低（p<0.01）。此外，MLAB组中普雷沃氏菌科_NK3B31属（p<0.01）和衣原体属（p = 0.03）的相对丰度较低。
总体而言，这些结果表明，虽然补充MLAB不能直接提高仔猪生长性能，但它有可能改善断奶仔猪的免疫功能并促进更健康的肠道微生物群，最终可能降低死亡率。
