Duddeck Karyn A, Petersen Tiffany E, Adkins Haley J, Smith Alexandra H, Hernandez Samantha, Wenner Seth J, Yao Dan, Chen Chi, Li Wenli, Fregulia Priscila, Larsen Anna, Jang Young Dal
Department of Animal and Food Science, University of Wisconsin-River Falls, River Falls, WI 54022, USA.
The ScienceHearted Center, Arm & Hammer Animal and Food Production, Waukesha, WI 53186, USA.
Animals (Basel). 2023 Dec 28;14(1):109. doi: 10.3390/ani14010109.
This experiment was conducted to evaluate the effects of dietary supplementation level of a two-strain probiotic on growth performance, blood parameters, fecal metabolites, and microbiome in nursery pigs. A total of 54 weaned piglets were allotted to three treatments in three replicate pens with six pigs/pen for a 28 d feeding trial. The treatments were as follows: control: no probiotic supplementation; Pro1x: supplementation at 1.875 × 10 CFU/g diet; and Pro10x: supplementation at 1.875 × 10 CFU/g diet. Body weight at d 14 postweaning ( = 0.06) and average daily gain for d 0 to 14 postweaning ( < 0.05) were greater in the Pro1x treatment than in the other treatments. Blood glucose levels were greater in both probiotic treatments than in the control treatment at d 14 postweaning ( < 0.05). In the fecal short-chain fatty acid (SCFA) concentrations, the butyrate concentrations were greater in the Pro1x treatment than in the other treatments ( < 0.05), and the acetate, propionate, and total SCFA concentrations were greater in the Pro1x treatment than in the Pro10x treatment ( < 0.05). The beta diversity of fecal microbiome composition at d 14 postweaning based on Unweighted Unifrac analysis was dissimilar between the Pro1x and Pro10x treatments ( < 0.05). In conclusion, dietary supplementation of two strains selected to reduce effects of pathogenic to nursery diets at 1.875 × 10 CFU/g diet improved the growth rate in the early postweaning period, increased fecal SCFA concentrations and altered the fecal microbial community composition. A higher dose of did not improve the performance parameters over those of the control piglets.
本试验旨在评估两株益生菌的日粮添加水平对保育猪生长性能、血液参数、粪便代谢产物和微生物群的影响。总共54头断奶仔猪被分配到三个处理组，每个处理组设三个重复栏，每栏6头猪，进行为期28天的饲养试验。处理组如下：对照组：不添加益生菌；Pro1x组：按1.875×10 CFU/g日粮添加；Pro10x组：按1.875×10 CFU/g日粮添加。断奶后第14天的体重（ = 0.06）和断奶后第0至14天的平均日增重（ < 0.05），Pro1x处理组高于其他处理组。断奶后第14天，两个益生菌处理组的血糖水平均高于对照组（ < 0.05）。在粪便短链脂肪酸（SCFA）浓度方面，Pro1x处理组的丁酸浓度高于其他处理组（ < 0.05），Pro1x处理组的乙酸、丙酸和总SCFA浓度高于Pro10x处理组（ < 0.05）。基于非加权Unifrac分析，断奶后第14天粪便微生物群组成的β多样性在Pro1x和Pro10x处理组之间存在差异（ < 0.05）。总之，日粮中添加两株选定的益生菌，以1.875×10 CFU/g日粮添加量降低病原菌对保育日粮的影响，可提高断奶后早期的生长速度，增加粪便SCFA浓度，并改变粪便微生物群落组成。较高剂量的益生菌并没有比对照仔猪更好地改善性能参数。
