Wang Tianwei, Teng Kunling, Liu Yayong, Shi Weixiong, Zhang Jie, Dong Enqiu, Zhang Xin, Tao Yong, Zhong Jin
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
School of Life Science, University of Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2019 Feb 5;10:90. doi: 10.3389/fmicb.2019.00090. eCollection 2019.
is a widespread bacterial species and is commonly used as a probiotic. PFM105 was isolated from the rectum of a healthy sow. Here we found that PFM105 showed probiotic effect on weaning piglets in which intestinal inflammation and unbalanced gut microbiota happened frequently. PFM105 was identified to improve the growth of weaning piglet and promote the development of small intestinal villi. Antibiotics are often used in weaning piglet to prevent intestinal infection and promote the growth of animal. We found that weaning piglets feeding with PFM105 showed similar growth promotion but decreased diarrhea incidence compared with those feeding with antibiotics. High-throughput sequencing was used to analyze the gut microbiota in weaning piglets treated with PFM105 or antibiotics. The relative abundance of beneficial microbes Prevotellaceae and Bifidobacteriaceae were increased in colon of weaning piglet feeding PFM105, while antibiotics increased the relative abundance of bacteria associated with pathogenicity, such as and . PFM 105 increased indicators of intestinal health including serum levels of IgM, IL-10, and TGF-β, and colonic levels of SCFAs. We found strong correlations between the alterations in gut microbiota composition caused by feeding antibiotics and probiotics and the measured growth and health parameters in weaning piglets. The addition of PFM105 could significantly increase the relative abundance of metabolic genes which may important to intestinal microbiota maturation. Altogether, we demonstrated here that PFM 105 could promote intestinal development through modulation of gut microbiota in weaning piglets.
是一种广泛存在的细菌物种，通常用作益生菌。PFM105是从一头健康母猪的直肠中分离出来的。在这里，我们发现PFM105对断奶仔猪具有益生菌作用，断奶仔猪经常发生肠道炎症和肠道微生物群失衡。已确定PFM105可促进断奶仔猪的生长并促进小肠绒毛的发育。抗生素常用于断奶仔猪以预防肠道感染并促进动物生长。我们发现，与喂食抗生素的断奶仔猪相比，喂食PFM105的断奶仔猪生长促进效果相似，但腹泻发生率降低。使用高通量测序分析用PFM105或抗生素处理的断奶仔猪的肠道微生物群。喂食PFM105的断奶仔猪结肠中有益微生物普雷沃氏菌科和双歧杆菌科的相对丰度增加，而抗生素增加了与致病性相关细菌的相对丰度，如和。PFM 105提高了肠道健康指标，包括血清IgM、IL-10和TGF-β水平以及结肠短链脂肪酸水平。我们发现，喂食抗生素和益生菌引起的肠道微生物群组成变化与断奶仔猪的生长和健康参数之间存在很强的相关性。添加PFM105可显著增加可能对肠道微生物群成熟很重要的代谢基因的相对丰度。总之，我们在此证明PFM 105可通过调节断奶仔猪的肠道微生物群促进肠道发育。
