Miglione Antonella, Raucci Ada, Mancini Marco, Gioia Valentina, Frugis Alessandro, Cinti Stefano
Department of Pharmacy, University of Naples 'Federico II', Via D. Montesano 49, 80131, Naples, Italy.
Department of Organic Micropollutants, Acea Infrastructure, Rome, 00191, Italy.
Talanta. 2025 Feb 1;283:127093. doi: 10.1016/j.talanta.2024.127093. Epub 2024 Oct 24.
One of the greatest challenges for the future is represented by the conservation of global agricultural production: it is necessary to implement early monitoring of the health conditions of crops through innovative technologies that allow a sustainable development. To reach this goal, the development of portable biosensors might represent a robust strategy for monitoring agricultural sites in agreement with the requirement of precision agriculture. In this work, an electrochemical inhibition biosensor has been engineered onto a glove to quantify organophosphorus pesticides directly on fruits peels. The biosensor was obtained through modification with a bio-hybrid probe which included Prussian blue, Carbon black and butyrylcholinesterase enzyme. The detection concept is based on the inhibition of the enzyme in presence of the pesticide, namely dichlorvos. The experimental setup has been characterized and the ultimate on-glove configuration has been applied towards the detection of dichlorvos directly on fruit peels, namely apples and oranges. The only task required for the end-user was to scrub the surface of the fruit with the strip and performing the electrochemical reading. The portable system was characterized by a low detection limit in the nanomolar range (high ppt) and a satisfactory repeatability lower than 10 %. It represents the starting point towards the design of on-glove biosensors in many fields of application, including the precision agriculture.
有必要通过允许可持续发展的创新技术对作物健康状况进行早期监测。为实现这一目标，便携式生物传感器的开发可能是一种符合精准农业要求的监测农业场地的有力策略。在这项工作中，一种电化学抑制生物传感器被设计在手套上，用于直接在水果表皮上定量检测有机磷农药。该生物传感器是通过用一种包含普鲁士蓝、炭黑和丁酰胆碱酯酶的生物杂交探针进行修饰而获得的。检测原理基于在农药（即敌敌畏）存在时酶的抑制作用。对实验装置进行了表征，并将最终的手套式配置应用于直接在苹果和橙子等水果表皮上检测敌敌畏。最终用户所需的唯一任务是用试纸擦拭水果表面并进行电化学读数。该便携式系统的特点是检测限低至纳摩尔范围（高皮克级），重复性令人满意，低于10%。它代表了在包括精准农业在内的许多应用领域设计手套式生物传感器的起点。
