Crop Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, Liaoning, China.
Liaoning Provincial Key Laboratory of Miscellaneous Grain Germplasm Innovation and Genetic Breeding, Liaoning Province, China.
Sci Rep. 2024 Nov 2;14(1):26413. doi: 10.1038/s41598-024-73936-1.
Mung bean (Vigna radiata L.), a widely cultivated legume, belongs to the Fabaceae family's Papilionoideae subfamily. Although Phosphatidylethanolamine-binding protein (PEBP) genes have been identified in several plant species, their presence and function in mung bean remain largely unexplored. In this study, we identified seven VrPEBP genes from mung bean and classified them into four clades: FT, MFT, TFL and FT-like. Cis-regulatory element analysis revealed that VrPEBP genes may play a role in light, hormone, and stress responses. Quantitative real-time PCR (qRT-PCR) analysis indicated that VrPEBPs were constitutively expressed in various tissues. However, tissue-specific expression patterns were observed among VrPEBP genes. Under short-day (SD) conditions, VrFT1 and VrMFT1 exhibited significantly higher expression levels than under long-day (LD) conditions at 8 and 4 h, respectively. Conversely, VrTFL2 and VrTFL3 showed significantly higher expression levels under LD conditions compared to SD conditions at 8 and 12 h, respectively. The varied expression patterns of these genes under different photoperiod suggest their potential involvement in the photoperiodic regulation of flowering in mung bean. Additionally, phenotypic analysis of transgenic Arabidopsis plants overexpressing VrFT1 revealed higher expression levels under SD conditions and predicted its role in promoting flowering. These results provide valuable insights into the evolution and function of PEBP genes in mung bean and lay the foundation for further research on their regulatory mechanisms and potential applications in mung bean improvement.
绿豆（Vigna radiata L.）是一种广泛种植的豆类，属于豆科蝶形花亚科。虽然已经在几种植物物种中鉴定出磷酯乙醇胺结合蛋白（PEBP）基因，但它们在绿豆中的存在和功能仍在很大程度上尚未得到探索。在本研究中，我们从绿豆中鉴定出了七个 VrPEBP 基因，并将它们分为四个分支：FT、MFT、TFL 和 FT-like。顺式作用元件分析表明，VrPEBP 基因可能在光、激素和应激反应中发挥作用。定量实时 PCR（qRT-PCR）分析表明，VrPEBPs 在各种组织中均有组成型表达。然而，VrPEBP 基因之间观察到组织特异性表达模式。在短日照（SD）条件下，VrFT1 和 VrMFT1 的表达水平分别在 8 小时和 4 小时时显著高于长日照（LD）条件下的表达水平。相反，VrTFL2 和 VrTFL3 在 LD 条件下的表达水平在 8 小时和 12 小时时分别显著高于 SD 条件下的表达水平。这些基因在不同光周期下的表达模式差异表明它们可能参与了绿豆的光周期开花调控。此外，过表达 VrFT1 的转基因拟南芥植株的表型分析表明，在 SD 条件下表达水平较高，并预测其在促进开花中的作用。这些结果为研究 PEBP 基因在绿豆中的进化和功能提供了有价值的见解，并为进一步研究其调控机制和在绿豆改良中的潜在应用奠定了基础。
