Laboratory of Genetic Breeding in Tomato, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture and Landscape Architecture, Northeast Agricultural University, Mucai Street 59, Harbin 150030, China.
Int J Mol Sci. 2023 May 24;24(11):9185. doi: 10.3390/ijms24119185.
The PEBP gene family is crucial for the growth and development of plants, the transition between vegetative and reproductive growth, the response to light, the production of florigen, and the reaction to several abiotic stressors. The PEBP gene family has been found in numerous species, but the SLPEBP gene family has not yet received a thorough bioinformatics investigation, and the members of this gene family are currently unknown. In this study, bioinformatics was used to identify 12 members of the SLPEBP gene family in tomato and localize them on the chromosomes. The physicochemical characteristics of the proteins encoded by members of the SLPEBP gene family were also examined, along with their intraspecific collinearity, gene structure, conserved motifs, and cis-acting elements. In parallel, a phylogenetic tree was built and the collinear relationships of the PEBP gene family among tomato, potato, pepper, and Arabidopsis were examined. The expression of 12 genes in different tissues and organs of tomato was analyzed using transcriptomic data. It was also hypothesized that , , , , , and might be related to tomato flowering and that , , , and might be related to ovary development based on the tissue-specific expression analysis of SLPEBP gene family members at five different stages during flower bud formation to fruit set. This article's goal is to offer suggestions and research directions for further study of tomato PEBP gene family members.
PEBP 基因家族对于植物的生长和发育、营养生长向生殖生长的转变、对光的响应、成花素的产生以及对多种非生物胁迫因子的反应至关重要。PEBP 基因家族在许多物种中都有发现，但 SLPEBP 基因家族尚未进行全面的生物信息学研究，目前尚不清楚该基因家族的成员。本研究利用生物信息学方法在番茄中鉴定了 12 个 SLPEBP 基因家族成员，并对它们在染色体上的位置进行了定位。还研究了 SLPEBP 基因家族成员编码蛋白的理化特性、种内共线性、基因结构、保守基元和顺式作用元件。同时，构建了系统发育树，并分析了番茄、马铃薯、辣椒和拟南芥中 PEBP 基因家族的共线性关系。利用转录组数据分析了 12 个基因在番茄不同组织和器官中的表达情况。根据花芽形成到果实形成过程中五个不同阶段的 SLPEBP 基因家族成员的组织特异性表达分析，推测 、 、 、 、 可能与番茄开花有关， 、 、 和 可能与子房发育有关。本文旨在为进一步研究番茄 PEBP 基因家族成员提供建议和研究方向。
