State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products; Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resource, Fujian Key Laboratory of Marine Genetic Resources, Xiamen, China.
Commun Biol. 2024 Nov 2;7(1):1428. doi: 10.1038/s42003-024-07079-4.
The widespread bacterial genus Sulfurimonas is metabolically versatile and occupies a key ecological niche in different habitats, but its interaction with bacteriophages remains unexplored. Here we systematically investigated the genetic diversity, taxonomy and interaction patterns of Sulfurimonas-associated phages based on sequenced microbial genomes and metagenomes. High-confidence phage contigs related to Sulfurimonas were retrieved from various ecosystems, clustered into 61 viral operational taxonomic units across three viral realms, including Duplodnaviria, Monodnaviria and Varidnaviria. Head-tail phages of Caudoviricetes were assigned to 19 genus-level viral clusters, the majority of which were distantly related to known viruses. Notably, diverse double jelly-roll viruses and inoviruses were also linked to Sulfurimonas, representing two commonly overlooked phage groups. Historical and current phage infections were revealed, implying viral impact on the evolution of host adaptive immunity. Additionally, phages carrying auxiliary metabolic genes might benefit hosts by compensating or augmenting sulfur metabolism. This study highlights the diversity and novelty of Sulfurimonas-associated phages with divergent tailless lineages, providing basis for further investigation of phage-host interactions within this genus.
广泛存在的细菌属 Sulfurimonas 具有代谢多样性，并在不同生境中占据关键的生态位，但它与噬菌体的相互作用仍未被探索。在这里，我们基于测序的微生物基因组和宏基因组，系统地研究了与 Sulfurimonas 相关的噬菌体的遗传多样性、分类和相互作用模式。从各种生态系统中检索到与 Sulfurimonas 相关的高可信度噬菌体基因序列，将其聚类为 61 个病毒分类操作单元，跨越三个病毒领域，包括 Duplodnaviria、Monodnaviria 和 Varidnaviria。Caudoviricetes 的头部-尾部噬菌体被分配到 19 个属水平的病毒聚类中，其中大多数与已知病毒的关系较远。值得注意的是，多样的双链结状病毒和肌尾病毒也与 Sulfurimonas 有关，代表了两个通常被忽视的噬菌体群体。历史和当前的噬菌体感染被揭示出来，暗示了病毒对宿主适应性免疫进化的影响。此外，携带辅助代谢基因的噬菌体可能通过补偿或增强硫代谢来使宿主受益。本研究突出了 Sulfurimonas 相关噬菌体的多样性和新颖性，具有不同的无尾谱系，为进一步研究该属内噬菌体-宿主相互作用提供了基础。
