Li Xiaofeng, Cheng Ruolin, Zhang Chuanxi, Shao Zongze
Ningbo University, Institute of Plant Virology, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo, China.
Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Ningbo, China.
Genet Mol Biol. 2024 Jul 29;47(2):e20230355. doi: 10.1590/1678-4685-GMB-2023-0355. eCollection 2024.
The globally widespread genus Sulfurimonas are playing important roles in different habitats, including the deep-sea hydrothermal vents. However, phages infecting Sulfurimonas have never been isolated and characterized to date. In the present study, a novel prophage SNW-1 was identified from Sulfurimonas indica NW79. Whole genome sequencing resulted in a circular, double-stranded DNA molecule of 37,096 bp with a mol% G+C content of 37. The genome includes 64 putative open reading frames, 33 of which code for proteins with predicted functions. Presence of hallmark genes associated with Caudoviricetes and genes involved in lysis and lysogeny indicated that SNW-1 should be a temperate, tailed phage. Phylogenetic and comparative proteomic analyses suggested that Sulfurimonas phage SNW-1 was distinct from other double stranded DNA phages and might represent a new viral genus.
在全球广泛分布的硫单胞菌属在包括深海热液喷口在内的不同生境中发挥着重要作用。然而，迄今为止，感染硫单胞菌的噬菌体尚未被分离和鉴定。在本研究中，从印度硫单胞菌NW79中鉴定出一种新型前噬菌体SNW-1。全基因组测序得到一个37096 bp的环状双链DNA分子，其G+C含量为37 mol%。该基因组包含64个推定的开放阅读框，其中33个编码具有预测功能的蛋白质。与长尾噬菌体目相关的标志性基因以及参与裂解和溶原性的基因的存在表明，SNW-1应该是一种温和的有尾噬菌体。系统发育和比较蛋白质组学分析表明，硫单胞菌噬菌体SNW-1与其他双链DNA噬菌体不同，可能代表一个新的病毒属。
