Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan, China.
State Key Laboratory of MicrobialTechnology, University, Qingdao, China.
Appl Environ Microbiol. 2024 Nov 20;90(11):e0122824. doi: 10.1128/aem.01228-24. Epub 2024 Nov 4.
Cra (catabolite repressor/activator) is a global transcription factor (TF) that plays a pleiotropic role in controlling the transcription of several genes involved in carbon utilization and energy metabolism. Multiple studies have investigated the regulatory mechanism of Cra and its rational use for metabolic regulation, but due to the complexity of its regulation, there remain challenges in the efficient use of Cra. Here, the structure, mechanism of action, and regulatory function of Cra in carbon and nitrogen flow are reviewed. In addition, this paper highlights the application of Cra in metabolic engineering, including the promotion of metabolite biosynthesis, the regulation of stress tolerance and virulence, the use of a Cra-based biosensor, and its coupling with other transcription factors. Finally, the prospects of Cra-related regulatory strategies are discussed. This review provides guidance for the rational design and construction of Cra-based metabolic regulation systems.
Cra（分解代谢物阻遏物/激活物）是一种全局转录因子（TF），在控制参与碳利用和能量代谢的几个基因的转录中发挥着多效性作用。多项研究已经调查了 Cra 的调节机制及其在代谢调节中的合理利用，但由于其调节的复杂性，在有效利用 Cra 方面仍然存在挑战。在这里，我们回顾了 Cra 在碳氮流中的结构、作用机制和调节功能。此外，本文还强调了 Cra 在代谢工程中的应用，包括促进代谢物生物合成、调节应激耐受性和毒力、使用基于 Cra 的生物传感器以及与其他转录因子的偶联。最后，讨论了与 Cra 相关的调控策略的前景。本综述为基于 Cra 的代谢调控系统的合理设计和构建提供了指导。
