大肠杆菌K-12中极性Tn5诱导的异亮氨酸-缬氨酸营养缺陷型的生理学特征：ilvOGEDA操纵子中存在内部启动子的证据
Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon.
作者信息
Berg C M, Shaw K J, Vender J, Borucka-Mankiewicz M
出版信息
Genetics. 1979 Oct;93(2):308-19.
Abstract
The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior, and enzyme activity. Mutants defective in ilvA, ilvC, ilvD and ilvE were found. Mutation in ilvE were not completely polar on ilvD and ilvA enzyme activities (that is, ilvE mutants possessed a low constitutive level of expression of the enzymes coded by ilvD and ilvA), while mutations in ilvD were completely polar on ilvA enzyme activity. The data suggest that there is an internal promoter between the sites of Tn5 insertion in ilvE and ilvD.
摘要
对转座子Tn5在大肠杆菌K-12中诱导产生的22种异亮氨酸-缬氨酸营养缺陷型菌株的特性，根据其生长需求、交叉补料行为和酶活性进行了表征。发现了ilvA、ilvC、ilvD和ilvE存在缺陷的突变体。ilvE中的突变对ilvD和ilvA酶活性并非完全呈极性影响（即ilvE突变体中由ilvD和ilvA编码的酶具有低组成型表达水平），而ilvD中的突变对ilvA酶活性完全呈极性影响。数据表明在ilvE和ilvD中Tn5插入位点之间存在一个内部启动子。
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