底物通道化：α-酮丁酸对鼠伤寒沙门氏菌乙酰羟酸合酶的抑制作用
Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium.
作者信息
Shaw K J, Berg C M
出版信息
J Bacteriol. 1980 Sep;143(3):1509-12. doi: 10.1128/jb.143.3.1509-1512.1980.
Abstract
Excess alpha-ketobutyrate inhibited the growth of Salmonella typhimurium LT2 by inhibiting the acetohydroxy acid synthase-catalyzed synthesis of alpha-acetolactate (a valine precursor). As a result, cells were starved for valine, and both ilvB (encoding acetohydroxy acid synthase I) and ilvGEDA (ilvG encodes acetohydroxy acid synthase II) were derepressed. The addition of valine reversed the effects of alpha-ketobutyrate.
摘要
过量的α-酮丁酸通过抑制乙酰羟酸合酶催化的α-乙酰乳酸（缬氨酸前体）合成来抑制鼠伤寒沙门氏菌LT2的生长。结果，细胞因缬氨酸缺乏而饥饿，ilvB（编码乙酰羟酸合酶I）和ilvGEDA（ilvG编码乙酰羟酸合酶II）均被去阻遏。添加缬氨酸可逆转α-酮丁酸的作用。
相似文献
1
Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium.底物通道化：α-酮丁酸对鼠伤寒沙门氏菌乙酰羟酸合酶的抑制作用
J Bacteriol. 1980 Sep;143(3):1509-12. doi: 10.1128/jb.143.3.1509-1512.1980.
2
Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.鼠伤寒沙门氏菌中乙酰羟酸合酶I和II缺陷型突变体。
J Bacteriol. 1980 Mar;141(3):1258-63. doi: 10.1128/jb.141.3.1258-1263.1980.
3
Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium.鼠伤寒沙门氏菌中支链氨基酸生物合成酶乙酰乳酸合酶的抑制导致α-酮丁酸的毒性积累。
J Bacteriol. 1987 Apr;169(4):1372-8. doi: 10.1128/jb.169.4.1372-1378.1987.
4
Regulation of expression of the ilvB operon in Salmonella typhimurium.鼠伤寒沙门氏菌中ilvB操纵子表达的调控
J Bacteriol. 1984 Dec;160(3):833-41. doi: 10.1128/jb.160.3.833-841.1984.
5
6
Acetohydroxy acid synthase I is required for isoleucine and valine biosynthesis by Salmonella typhimurium LT2 during growth on acetate or long-chain fatty acids.鼠伤寒沙门氏菌LT2在以乙酸盐或长链脂肪酸为碳源生长时，异亮氨酸和缬氨酸的生物合成需要乙酰羟酸合酶I。
J Bacteriol. 1987 Feb;169(2):917-9. doi: 10.1128/jb.169.2.917-919.1987.
7
Metabolic effects of inhibitors of two enzymes of the branched-chain amino acid pathway in Salmonella typhimurium.鼠伤寒沙门氏菌中支链氨基酸途径两种酶抑制剂的代谢效应
J Bacteriol. 1996 Feb;178(4):1187-96. doi: 10.1128/jb.178.4.1187-1196.1996.
8
IlvHI locus of Salmonella typhimurium.鼠伤寒沙门氏菌的ilvHI基因座。
J Bacteriol. 1983 Jun;154(3):1054-63. doi: 10.1128/jb.154.3.1054-1063.1983.
9
Leaky pantothenate and thiamin mutations of Salmonella typhimurium conferring suphometuron methyl sensitivity.鼠伤寒沙门氏菌的泛酸和硫胺素渗漏突变赋予其对甲磺隆的敏感性。
J Gen Microbiol. 1989 Aug;135(8):2209-22. doi: 10.1099/00221287-135-8-2209.
10
Sensitivity of a Salmonella typhimurium aspC mutant to sulfometuron methyl, a potent inhibitor of acetolactate synthase II.鼠伤寒沙门氏菌aspC突变体对磺酰脲类甲基（乙酰乳酸合酶II的有效抑制剂）的敏感性。
J Bacteriol. 1986 Feb;165(2):386-92. doi: 10.1128/jb.165.2.386-392.1986.
引用本文的文献
1
Moonlighting activity of threonine synthase in cyanobacterial cell death.苏氨酸合酶在蓝藻细胞死亡中的兼职活动。
mSystems. 2025 Jun 17;10(6):e0031025. doi: 10.1128/msystems.00310-25. Epub 2025 May 5.
2
Conserved pyridoxal protein that regulates Ile and Val metabolism.调节异亮氨酸和缬氨酸代谢的保守吡哆醛蛋白。
J Bacteriol. 2013 Dec;195(24):5439-49. doi: 10.1128/JB.00593-13. Epub 2013 Oct 4.
3
Physiology of pyruvate metabolism in Lactococcus lactis.乳酸乳球菌中丙酮酸代谢的生理学
Antonie Van Leeuwenhoek. 1996 Oct;70(2-4):253-67. doi: 10.1007/BF00395936.
4
Metabolic basis for the isoleucine, pantothenate or methionine requirement of ilvG strains of Salmonella typhimurium.鼠伤寒沙门氏菌ilvG菌株对异亮氨酸、泛酸盐或蛋氨酸需求的代谢基础。
J Bacteriol. 1982 Jun;150(3):1202-11. doi: 10.1128/jb.150.3.1202-1211.1982.
5
Organization and regulation of the ilvGEDA operon in Salmonella typhimurium LT2.鼠伤寒沙门氏菌LT2中ilvGEDA操纵子的组织与调控
J Bacteriol. 1981 Feb;145(2):984-9. doi: 10.1128/jb.145.2.984-989.1981.
6
Hydroxyamino acid utilization and alpha-ketobutyrate toxicity in Pseudomonas cepacia.洋葱伯克霍尔德菌中羟基氨基酸的利用及α-酮丁酸毒性
J Bacteriol. 1980 Oct;144(1):441-3. doi: 10.1128/jb.144.1.441-443.1980.
7
Linkage map of Salmonella typhimurium, Edition VI.鼠伤寒沙门氏菌连锁图谱，第六版。
Microbiol Rev. 1983 Sep;47(3):410-53. doi: 10.1128/mr.47.3.410-453.1983.
8
Involvement of ack-pta operon products in alpha-ketobutyrate metabolism by Salmonella typhimurium.鼠伤寒沙门氏菌中ack-pta操纵子产物参与α-酮丁酸代谢
Mol Gen Genet. 1987 May;207(2-3):435-40. doi: 10.1007/BF00331612.
9
Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium.鼠伤寒沙门氏菌中支链氨基酸生物合成酶乙酰乳酸合酶的抑制导致α-酮丁酸的毒性积累。
J Bacteriol. 1987 Apr;169(4):1372-8. doi: 10.1128/jb.169.4.1372-1378.1987.
10
Sensitivity of a Salmonella typhimurium aspC mutant to sulfometuron methyl, a potent inhibitor of acetolactate synthase II.鼠伤寒沙门氏菌aspC突变体对磺酰脲类甲基（乙酰乳酸合酶II的有效抑制剂）的敏感性。
J Bacteriol. 1986 Feb;165(2):386-92. doi: 10.1128/jb.165.2.386-392.1986.
本文引用的文献
1
Isoleucine and valine metabolism in Escherichia coli. X. The enzymatic formation of acetohydroxybutyrate.大肠杆菌中异亮氨酸和缬氨酸的代谢。X. 乙酰羟丁酸的酶促形成。
J Biol Chem. 1961 Sep;236:2486-91.
2
Improved synthesis of the sodium salt of DL-alpha, beta-dihydroxyisovaleric acid.DL-α,β-二羟基异戊酸钠盐的改进合成方法。
Anal Biochem. 1980 May 15;104(2):485-8. doi: 10.1016/0003-2697(80)90104-9.
3
Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.鼠伤寒沙门氏菌中乙酰羟酸合酶I和II缺陷型突变体。
J Bacteriol. 1980 Mar;141(3):1258-63. doi: 10.1128/jb.141.3.1258-1263.1980.
4
Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase.大肠杆菌中异亮氨酸和缬氨酸的代谢。XX. 乙酰羟酸合成酶的多种形式。
Biochem Biophys Res Commun. 1972 Jul 25;48(2):444-50. doi: 10.1016/s0006-291x(72)80071-8.
5
Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium.鼠伤寒沙门氏菌中生物合成乙酰羟酸合成酶的两种形式。
Biochem Biophys Res Commun. 1972 Jul 25;48(2):437-43. doi: 10.1016/s0006-291x(72)80070-6.
6
Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.通过插入携带反向重复序列的耐药元件进行诱变。
J Mol Biol. 1975 Oct 5;97(4):561-75. doi: 10.1016/s0022-2836(75)80059-3.
7
8
Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12.影响大肠杆菌K-12中乙酰羟酸合酶II形成的突变
Mol Gen Genet. 1979 Feb 1;169(3):299-314. doi: 10.1007/BF00382276.
Zotero 插件