Menken B Z, Su L C, Ayaz K L, Csallany A S
J Nutr. 1986 Mar;116(3):350-5. doi: 10.1093/jn/116.3.350.
The effect of age and dietary supplementation of vitamin E or N,N'-diphenyl-p-phenylenediamine (DPPD) on organic solvent-soluble lipofuscin pigments (OLP) and glutathione peroxidase (GSH-Px) activity in mouse heart and brain was investigated. Four groups of 32 female weanling mice were fed a basal diet containing either 0, 30 or 300 ppm RRR-alpha-tocopheryl acetate (d-alpha-tocopheryl acetate) or 30 ppm DPPD from 2 to 18 mo of age. Neither GSH-Px activity nor dietary supplementation of vitamin E or DPPD had an effect on OLP concentrations in the brain or heart. OLP levels were two- to fourfold higher at 12 mo of age in the heart and were lower at 18 mo of age in the brain than at 2 or 9 mo of age. GSH-Px activity increased with age in the heart tissue of vitamin E-deficient and DPPD-supplemented mice only. No change in GSH-Px activity was observed in the brain due to diet or increasing age. These results suggested that OLP concentrations were not affected by dietary supplementation of vitamin E or DPPD but were affected by age-related factors in the mouse brain and heart.
研究了年龄以及维生素E或N，N'-二苯基对苯二胺（DPPD）膳食补充对小鼠心脏和大脑中有机溶剂可溶性脂褐素色素（OLP）和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。四组32只雌性断奶小鼠从2至18月龄开始喂食含有0、30或300 ppm RRR-α-生育酚醋酸酯（d-α-生育酚醋酸酯）或30 ppm DPPD的基础日粮。GSH-Px活性以及维生素E或DPPD的膳食补充对大脑或心脏中的OLP浓度均无影响。心脏中12月龄时的OLP水平比2或9月龄时高两至四倍，而大脑中18月龄时的OLP水平比2或9月龄时低。仅在维生素E缺乏和补充DPPD的小鼠心脏组织中，GSH-Px活性随年龄增加。未观察到饮食或年龄增长导致大脑中GSH-Px活性发生变化。这些结果表明，OLP浓度不受维生素E或DPPD膳食补充的影响，但受小鼠大脑和心脏中与年龄相关因素的影响。
