小鼠脑和心脏中的有机溶剂可溶性脂褐素色素及谷胱甘肽过氧化物酶：年龄和维生素E的影响

Organic solvent-soluble lipofuscin pigments and glutathione peroxidase in mouse brain and heart: effects of age and vitamin E.

作者信息

Menken B Z, Su L C, Ayaz K L, Csallany A S

出版信息

J Nutr. 1986 Mar;116(3):350-5. doi: 10.1093/jn/116.3.350.

DOI:10.1093/jn/116.3.350

Abstract

The effect of age and dietary supplementation of vitamin E or N,N'-diphenyl-p-phenylenediamine (DPPD) on organic solvent-soluble lipofuscin pigments (OLP) and glutathione peroxidase (GSH-Px) activity in mouse heart and brain was investigated. Four groups of 32 female weanling mice were fed a basal diet containing either 0, 30 or 300 ppm RRR-alpha-tocopheryl acetate (d-alpha-tocopheryl acetate) or 30 ppm DPPD from 2 to 18 mo of age. Neither GSH-Px activity nor dietary supplementation of vitamin E or DPPD had an effect on OLP concentrations in the brain or heart. OLP levels were two- to fourfold higher at 12 mo of age in the heart and were lower at 18 mo of age in the brain than at 2 or 9 mo of age. GSH-Px activity increased with age in the heart tissue of vitamin E-deficient and DPPD-supplemented mice only. No change in GSH-Px activity was observed in the brain due to diet or increasing age. These results suggested that OLP concentrations were not affected by dietary supplementation of vitamin E or DPPD but were affected by age-related factors in the mouse brain and heart.

摘要

研究了年龄以及维生素E或N，N'-二苯基对苯二胺（DPPD）膳食补充对小鼠心脏和大脑中有机溶剂可溶性脂褐素色素（OLP）和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。四组32只雌性断奶小鼠从2至18月龄开始喂食含有0、30或300 ppm RRR-α-生育酚醋酸酯（d-α-生育酚醋酸酯）或30 ppm DPPD的基础日粮。GSH-Px活性以及维生素E或DPPD的膳食补充对大脑或心脏中的OLP浓度均无影响。心脏中12月龄时的OLP水平比2或9月龄时高两至四倍，而大脑中18月龄时的OLP水平比2或9月龄时低。仅在维生素E缺乏和补充DPPD的小鼠心脏组织中，GSH-Px活性随年龄增加。未观察到饮食或年龄增长导致大脑中GSH-Px活性发生变化。这些结果表明，OLP浓度不受维生素E或DPPD膳食补充的影响，但受小鼠大脑和心脏中与年龄相关因素的影响。