Csallany A S, Su L C, Menken B Z
J Nutr. 1984 Sep;114(9):1582-7. doi: 10.1093/jn/114.9.1582.
The present experiment was designed to investigate the effect of selenium (Se) supplementation, as sodium selenite, on organic solvent-soluble lipofuscin pigment (OLP) accumulation and glutathione peroxidase (GSH-Px) activity in the livers of mice fed varying levels of vitamin E or N,N'-diphenyl-p-phenylenediamine (DPPD). Four groups of 16 female, weanling mice each were fed either a vitamin E-deficient diet, a diet supplemented with 30 mg/kg or 300 mg/kg vitamin E (as RRR-alpha-tocopheryl acetate), or a diet supplemented with 30 mg/kg DPPD. Each diet contained 0.05 ppm Se. At 5 months of age, eight animals from each dietary group were supplemented with an additional 0.1 ppm Se, as sodium selenite, in their drinking water. The remaining animals were fed their original diets through the 9-month experimental period. Selenite supplementation resulted in a significant increase in OLP concentration and GSH-Px activity in the liver of mice fed vitamin E- or DPPD-supplemented diets. Normal levels of vitamin E and DPPD (30 mg/kg) were not sufficient to protect against the oxidative effects of selenite; however, 10 times the normal level of vitamin E (300 mg/kg) markedly suppressed this oxidative effect.
本实验旨在研究以亚硒酸钠形式补充硒对喂食不同水平维生素E或N，N'-二苯基对苯二胺（DPPD）的小鼠肝脏中有机溶剂可溶性脂褐素色素（OLP）积累和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。将四组每组16只雌性断奶小鼠分别喂食维生素E缺乏饮食、补充30 mg/kg或300 mg/kg维生素E（作为RRR-α-生育酚乙酸酯）的饮食或补充30 mg/kg DPPD的饮食。每种饮食含0.05 ppm硒。在5月龄时，每个饮食组的8只动物在饮用水中额外补充0.1 ppm亚硒酸钠形式的硒。其余动物在9个月的实验期内喂食其原始饮食。补充亚硒酸钠导致喂食补充维生素E或DPPD饮食的小鼠肝脏中OLP浓度和GSH-Px活性显著增加。正常水平的维生素E和DPPD（30 mg/kg）不足以抵御亚硒酸钠的氧化作用；然而，维生素E正常水平的10倍（300 mg/kg）显著抑制了这种氧化作用。
