Ibrahim Abed Alqader, Khan Tariq, Nowlin Kyle, Averitt Jared, Pathiraja Gayani, LaJeunesse Dennis, Obare Sherine O, Dellinger Anthony L
Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro Greensboro NC 27401 USA
Kepley Biosystems Incorporated Greensboro NC 27214 USA.
Nanoscale Adv. 2024 Nov 4;6(23):5833-52. doi: 10.1039/d4na00732h.
Nanoparticle modification demonstrates a remarkable synergetic effect in combating bacteria, particularly resistant bacteria, enhancing their efficacy by simultaneously targeting multiple cellular pathways. This approach positions them as a potent solution against the growing challenge of antimicrobial-resistant (AMR) strains. This research presents an investigation into the synthesis, characterization, and antibacterial evaluation of silver-coordinated chloro-fullerenes nanoparticles (Ag-C-Cl) and copper-coordinated chloro-fullerenes nanoparticles (Cu-C-Cl). Utilizing an innovative, rapid one-step synthesis approach, the nanoparticles were rigorously characterized using X-ray Photoelectron Spectroscopy (XPS), Scanning Electron Microscopy-Energy Dispersive X-ray Spectrometer (SEM-EDS), High-Resolution Transmission Electron Microscopy (HR-TEM), Fourier-Transform Infrared Spectroscopy (FTIR), and Raman spectroscopy. In conjunction with the analytical techniques, a computational approach was utilized to corroborate the findings from Raman spectroscopy as well as the surface potential of these nanoparticles. Moreover, the antibacterial activities of the synthesized nanoparticles were assessed against () and Methicillin-Resistant (MRSA). These findings demonstrated that the synthesized Ag-C-Cl and Cu-C-Cl nanoparticles exhibited minimum inhibitory concentrations (MIC) of 3.9 μg mL and 125 μg mL, respectively. Reactive oxygen species (ROS) quantification, catalase assay, and efflux pump inhibition results revealed promising broad-spectrum antibacterial effects.
纳米颗粒修饰在对抗细菌，特别是耐药细菌方面显示出显著的协同效应，通过同时靶向多个细胞途径提高其功效。这种方法使其成为应对抗微生物耐药（AMR）菌株日益增长挑战的有效解决方案。本研究对银配位氯富勒烯纳米颗粒（Ag-C-Cl）和铜配位氯富勒烯纳米颗粒（Cu-C-Cl）的合成、表征及抗菌性能进行了研究。采用创新的快速一步合成方法，利用X射线光电子能谱（XPS）、扫描电子显微镜-能量色散X射线光谱仪（SEM-EDS）、高分辨率透射电子显微镜（HR-TEM）、傅里叶变换红外光谱（FTIR）和拉曼光谱对纳米颗粒进行了严格表征。结合分析技术，采用计算方法来证实拉曼光谱的结果以及这些纳米颗粒的表面电位。此外，评估了合成纳米颗粒对（）和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性。这些结果表明，合成的Ag-C-Cl和Cu-C-Cl纳米颗粒的最低抑菌浓度（MIC）分别为3.9μg/mL和125μg/mL。活性氧（ROS）定量、过氧化氢酶测定和外排泵抑制结果显示出有前景的广谱抗菌效果。
