Ata Emre, Özgüç Semiha, Temel Mustafa Hüseyin, Beyaztaş Hakan, Aktaş Selman, Güler Eray Metin
Department of Physical Medicine and Rehabilitation, Sultan 2. Abdulhamid Han Training and Research Hospital, İstanbul, Türkiye.
Department of Physical Medicine and Rehabilitation, Üsküdar State Hospital, İstanbul, Türkiye.
Arch Rheumatol. 2024 Aug 26;39(3):459-466. doi: 10.46497/ArchRheumatol.2024.10375. eCollection 2024 Sep.
This study aimed to examine the effect of varying ozone doses on proinflammatory cytokine levels in the synovial fluid collected from individuals with knee osteoarthritis.
The controlled clinical trial was conducted with 82 patients (61 females, 21 males; mean age: 63.1±10.0 years; range, 40 to 73 years) between 21 April 2023 and 20 May 2023. Synovial fluid samples were collected from the patients under ultrasound guidance and divided into three tubes, one of which was not injected with ozone, and the other two were injected with 10 and 30 gamma (γ) ozone, respectively. The total antioxidant status, total oxidant status, oxidative stress index, interleukin (IL)-1 beta (β), IL-6, and tumor necrosis factor-alpha (TNF-α) in the joint fluids were measured.
The oxidative stress index and IL-1 β, IL-6, and TNF-α levels in the synovial fluid were lower at 10 and 30 γ compared to 0 and 10 γ, respectively. ozone injection at 30 gamma was more effective in reducing proinflammatory cytokines in the synovial fluid than that at 10 and 0 γ. Ozone injection into the pathological joint fluid was more effective in terms of total antioxidant status at 10 and 30 γ compared to 0 and 10 γ, respectively. No significant difference in total oxidant status was observed between the groups.
This study showed that ozone injection at 30 γ was more effective in reducing proinflammatory cytokines in the synovial fluid and in improving total antioxidant status than that at 10 and 0 γ. The results showed the potential significance of the ozone injection dosage in treating knee osteoarthritis.
本研究旨在探讨不同臭氧剂量对从膝骨关节炎患者采集的滑液中促炎细胞因子水平的影响。
2023年4月21日至2023年5月20日期间，对82例患者（61例女性，21例男性；平均年龄：63.1±10.0岁；范围40至73岁）进行了对照临床试验。在超声引导下从患者采集滑液样本，并分为三管，其中一管未注射臭氧，另外两管分别注射10和30伽马（γ）的臭氧。测量关节液中的总抗氧化状态、总氧化状态、氧化应激指数、白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α（TNF-α）。
与0和10γ相比，10和30γ时滑液中的氧化应激指数以及IL-1β、IL-6和TNF-α水平较低。30伽马的臭氧注射在降低滑液中促炎细胞因子方面比10和0γ更有效。与0和10γ相比，10和30γ时向病理性关节液中注射臭氧在总抗氧化状态方面更有效。各组之间总氧化状态未观察到显著差异。
本研究表明，30γ的臭氧注射在降低滑液中促炎细胞因子和改善总抗氧化状态方面比10和0γ更有效。结果显示了臭氧注射剂量在治疗膝骨关节炎中的潜在意义。
