"Check Up-Day Surgery" Polydiagnostic and Research Laboratory, Andrology Unit, Salerno, Italy.
"D'Arena" Clinical Analysis and Diagnostics Laboratory, Salerno, Italy.
Sci Rep. 2024 Nov 9;14(1):27422. doi: 10.1038/s41598-024-79047-1.
Male infertility is a complex issue influenced by multiple environmental and pathological factors. In this context, the impact of Human papillomavirus (HPV) infection on male fertility remains controversial. The introduction of new WHO 2021 evaluation criteria, included in the 6th ed. of Laboratory Manual for the examination and processing of human semen, i.e. DNA fragmentation index (DFI), slow and rapid progressive motility, could provide additional information about this correlation. 121 semen samples of male partners of HPV-positive women attending In Vitro Fertilization (IVF) were evaluated following WHO 2021 and HPV-DNA test. Comparing HPV-negative and positive samples for rapid and slow progressive motility showed significantly different results (p = 0.0018, p = 0.0004), contrary to what was observed for total progressive motility. Regarding sperm DFI, only high-risk HPV infections affected DNA integrity. In addition, the correlation between the different semen parameters revealed a significant correlation between midpiece morphological defects and rapid progressive motility in the HPV-positive group (rho = 0.43, p = 0.0006). In conclusion, WHO 2021 provides additional information regarding HPV's impact on seminal parameters. The correlation between HPV positivity, midpiece defects and a higher rapid progressive motility opens new research perspectives that may help unravel the issues surrounding the role of HPV in compromising sperm quality.
男性不育是一个受多种环境和病理因素影响的复杂问题。在这种情况下，人乳头瘤病毒（HPV）感染对男性生育力的影响仍存在争议。新的世卫组织 2021 年评估标准的引入，包括在第六版《人类精液检查与处理实验室手册》中，即 DNA 碎片化指数（DFI）、缓慢和快速前向运动，可能为这种相关性提供更多信息。对 121 名 HPV 阳性女性接受体外受精（IVF）的男性伴侣的精液样本按照世卫组织 2021 年和 HPV-DNA 检测标准进行了评估。比较快速和缓慢前向运动的 HPV 阴性和阳性样本显示出显著不同的结果（p=0.0018，p=0.0004），与总前向运动的结果相反。关于精子 DFI，只有高危 HPV 感染会影响 DNA 完整性。此外，不同精液参数之间的相关性显示 HPV 阳性组中段形态缺陷与快速前向运动之间存在显著相关性（rho=0.43，p=0.0006）。总之，世卫组织 2021 年提供了关于 HPV 对精液参数影响的更多信息。HPV 阳性、中段缺陷和较高的快速前向运动之间的相关性开辟了新的研究视角，可能有助于揭示 HPV 损害精子质量的作用所涉及的问题。
