Yu Shuai, Ma Shengtao, Wang Qiuling, Chen Zhifei, Xi Gaolei, An Nan, Yao Hanning, Jia Tao, Zhao Xiuhua, Yang Lei
Key Laboratory of Forest Plant Ecology, Ministry of Education, Engineering Research Center of Forest Bio-Preparation, College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, China.
Technology Center, China Tobacco Henan Industrial Limited Company, Zhengzhou, China.
Phytochem Anal. 2025 Apr;36(3):819-831. doi: 10.1002/pca.3470. Epub 2024 Nov 12.
This study investigates the composition and antibacterial properties of essential oil extracted from Pinus koraiensis (Siebold & Zucc) pine needles using a liquid nitrogen freezing treatment combined with solvent-free microwave extraction (LNSFM).
The aim is to develop a low-energy, high-efficiency extraction method for conifer essential oils, analyze their chemical composition, and evaluate their antibacterial efficacy.
Pine needle samples were frozen with liquid nitrogen and subsequently crushed. The essential oil was extracted using solvent-free microwave technology. A single-factor test and response surface methodology were employed to optimize extraction parameters. The extraction efficiency of LNSFM was compared with traditional methods through kinetics, and the essential oil components were analyzed using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity of the extracted volatile oil was tested against Escherichia coli and Staphylococcus aureus.
LNSFM proves to be a green and efficient extraction method suitable for obtaining volatile oils from pine needles, which demonstrate significant antibacterial properties.
本研究调查了采用液氮冷冻处理结合无溶剂微波萃取法（LNSFM）从红松（Siebold & Zucc）针叶中提取的精油的成分和抗菌特性。
旨在开发一种用于针叶树精油的低能耗、高效提取方法，分析其化学成分，并评估其抗菌功效。
将松针样品用液氮冷冻，随后粉碎。使用无溶剂微波技术提取精油。采用单因素试验和响应面法优化提取参数。通过动力学将LNSFM的提取效率与传统方法进行比较，并使用气相色谱-质谱联用仪（GC-MS）分析精油成分。测试提取的挥发油对大肠杆菌和金黄色葡萄球菌的抗菌活性。
LNSFM被证明是一种绿色高效的提取方法，适用于从松针中获取挥发油，这些挥发油具有显著的抗菌特性。
