Bawari Sweta, Sah Archana N, Gupta Pawan, Zengin Gökhan, Tewari Devesh
Amity Institute of Pharmacy, Amity University Campus, Sector-125, Noida, 201313, Gautam Buddha Nagar, Uttar Pradesh, India.
Department of Pharmaceutical Sciences, Faculty of Technology, Bhimtal, Kumaun University, Nainital, Uttarakhand, 263136, India.
J Ethnopharmacol. 2023 Apr 24;306:116157. doi: 10.1016/j.jep.2023.116157. Epub 2023 Jan 13.
Citrus fruits are a very rich source of electrolytes and citric acid. They have been used traditionally for treating urinary ailments and renal stones. Citrus jambhiri is indigenously used as a diuretic.
Present study aimed at establishing the antiurolithiatic potential of the juice of Citrus jambhiri fruits along with the elucidation of the mechanism involved in the urolithiasis disease defying activity.
The antiurolithiatic activity was established by means of nucleation, growth and aggregation assay in the in vitro settings and by means of ethylene glycol mediated calcium oxalate urolithiasis in the male Wistar rats. Docking studies were performed in an attempt to determine the mechanism of the antiurolithiatic action.
Present study revealed the role of C. jambhiri fruit juice in reducing nucleation, growth and aggregation of calcium oxalate crystals by possible reduction in the urinary supersaturation relative to calcium oxalate and raising the zeta potential of the calcium oxalate crystals. C. jambhiri fruit juice treatment in experimental rats produced significant amelioration of hypercalciuria, hyperoxaluria, hyperphosphaturia, hyperproteinuria, hyperuricosuria, hypocitraturia and hypomagnesiuria and ion activity product of calcium oxalate. It exhibited nephroprotection against calcium oxalate crystals induced renal tubular dilation and renal tissue deterioration. Docking studies further revealed high binding potential of the phytoconstituents of C. jambhiri viz. narirutin, neohesperidin, hesperidin, rutin and citric acid with glycolate oxidase and matrix metalloproteinase-9.
C. jambhiri fruit juice possesses excellent antiurolithiatic activity. The study reveals antiurolithiatic mechanism that involves restoration of equilibrium between the promoters and inhibitors of stone formation; and inhibition of matrix metalloproteinases and glycolate oxidase.
柑橘类水果是电解质和柠檬酸的丰富来源。它们传统上用于治疗泌尿系统疾病和肾结石。酸橙在当地被用作利尿剂。
本研究旨在确定酸橙果实汁液的抗尿路结石潜力，并阐明其抗尿路结石疾病活性所涉及的机制。
通过体外成核、生长和聚集试验以及雄性Wistar大鼠中乙二醇介导的草酸钙尿路结石模型来确定抗尿路结石活性。进行对接研究以确定抗尿路结石作用的机制。
本研究揭示了酸橙果实汁液通过可能降低相对于草酸钙的尿液过饱和度以及提高草酸钙晶体的ζ电位来减少草酸钙晶体的成核、生长和聚集。在实验大鼠中，酸橙果实汁液治疗显著改善了高钙尿症、高草酸尿症、高磷尿症、高蛋白尿症、高尿酸尿症、低枸橼酸尿症和低镁尿症以及草酸钙的离子活性产物。它对草酸钙晶体诱导的肾小管扩张和肾组织恶化具有肾保护作用。对接研究进一步揭示了酸橙的植物成分，即橙皮苷、新橙皮苷、橙皮苷、芦丁和柠檬酸与乙醇酸氧化酶和基质金属蛋白酶-9具有高结合潜力。
酸橙果实汁液具有优异的抗尿路结石活性。该研究揭示了抗尿路结石机制，包括恢复结石形成促进剂和抑制剂之间的平衡；以及抑制基质金属蛋白酶和乙醇酸氧化酶。
