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通过非接触电容耦合静电场增强细胞迁移能力。

Enhancing cell motility via non-contact capacitively coupled electrostatic field.

机构信息

Department of Physics and Astronomy (DIFA), Alma Mater Studiorum University of Bologna, Viale Berti Pichat 6/2, Bologna, 40127, Italy.

National Institute for Nuclear Physics (INFN BO), Bologna section, Viale Berti Pichat 6/2, Bologna, 40127, Italy.

出版信息

Sci Rep. 2024 Nov 15;14(1):28085. doi: 10.1038/s41598-024-77384-9.

DOI:10.1038/s41598-024-77384-9
PMID:39543219
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11564694/
Abstract

Cellular motility is essential for making and maintaining multicellular organisms throughout their lifespan. Migrating cells can move either individually or collectively by a crawling movement that links the cytoskeletal activity to the adhesion surface. In vitro stimulation by electric fields can be achieved by direct, capacitive or inductive coupled setups. We tested the effects of electrical stimulation provided by capacitive coupling on glioma cells, using a capacitive-coupled system powered by a potential difference of 35 V between two electrodes placed outside the culture dish. Numerical dosimetry identified two different fields: (i) in the order of 10 V/m at the level of the dielectric substrates, with almost uniform distribution; (ii) in the order of 10 V/m at the level of the culture medium, with spatial and material-dependent distribution. The scratch assay and the tracking of single-cell movement showed a boosted motility when crawling occurs on polystyrene surfaces, demonstrating the feasibility of this peculiar exposure system to generate forces capable of influencing cell behavior.

摘要

细胞运动对于多细胞生物在其整个生命周期中的形成和维持至关重要。迁移细胞可以通过与粘附表面相连的爬行运动单独或集体移动。通过直接、电容或感应耦合设置可以在体外刺激电场。我们使用由两个置于培养皿外的电极之间的 35 V 电位差供电的电容耦合系统，测试了电容耦合提供的电刺激对神经胶质瘤细胞的影响。数值剂量学确定了两种不同的场：（i）在介电基底的量级为 10 V/m，分布几乎均匀；（ii）在培养介质的量级为 10 V/m，分布具有空间和材料依赖性。划痕试验和单细胞运动的跟踪显示，当在聚苯乙烯表面爬行时，运动性增强，表明这种特殊的暴露系统产生能够影响细胞行为的力的可行性。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/11564694/ad9034f68cb6/41598_2024_77384_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/11564694/41a6db32ee6e/41598_2024_77384_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/11564694/e64d8e65f979/41598_2024_77384_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/11564694/912a0a2936f0/41598_2024_77384_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5461/11564694/ca564e4d7561/41598_2024_77384_Fig9_HTML.jpg
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