Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang Provincial Key Laboratory of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Zhejiang University, Hangzhou, 310009, P.R. China.
Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, P.R. China.
J Nanobiotechnology. 2024 Nov 15;22(1):713. doi: 10.1186/s12951-024-02985-5.
Bacterial infections, especially induced by multidrug-resistant pathogens, have become a significant global health concern. In the infected tissues, biofilms not only serve as a source of nutrients but also act as protective barriers that impede antibiotic penetration. Herein, we developed tea polyphenols epigallocatechin gallate (EGCG) Au nanoparticles (E-Au NPs) through direct one-step self-assembly methods by EGCG chelating with Au ions to eradicate antibiotic-resistant bacteria methicillin-resistant Staphylococcus aureus (MRSA) and prevent the formation of biofilm under near-infrared (NIR) irradiation. The outstanding antibacterial effect involved in mild photothermal therapy, reactive oxygen species production, pathogenicity-related genes regulation, and quinoprotein formation that were specific to the polyphenol-based NPs. The excellent antibacterial and anti-inflammatory therapeutic efficacy of E-Au NPs was validated and topically applied in murine MRSA-infected skin wounds and keratitis model in vivo to kill bacteria, reduce the inflammation response and promote wound healing. Furthermore, the ophthalmic and systemic biosafety profiles were thoroughly evaluated while no significant side effects were revealed achieving a balance between high-efficiency antibacterial properties and biocompatibility. This study provides an effective therapeutic agent of metal-phenolic materials for superficial tissue infection with favorable prognosis and potential in clinical translation.
细菌感染，特别是由多药耐药病原体引起的感染，已成为一个重大的全球健康问题。在感染组织中，生物膜不仅是营养物质的来源，而且还起到保护屏障的作用，阻碍抗生素的渗透。在此，我们通过 EGCG 与 Au 离子的直接一步自组装方法，开发了茶多酚表没食子儿茶素没食子酸酯（EGCG）金纳米粒子（E-Au NPs），以消除耐抗生素的金黄色葡萄球菌（MRSA）并防止在近红外（NIR）照射下形成生物膜。这种出色的抗菌作用涉及温和的光热疗法、活性氧物质的产生、致病性相关基因的调控以及多酚类 NPs 特有的醌蛋白形成。E-Au NPs 的优异抗菌和抗炎治疗效果在体内 MRSA 感染的小鼠皮肤伤口和角膜炎模型中得到了验证和局部应用，以杀死细菌、减少炎症反应并促进伤口愈合。此外，还对眼科和全身生物安全性概况进行了彻底评估，未发现明显的副作用，实现了高效抗菌性能和生物相容性之间的平衡。这项研究为浅表组织感染提供了一种有效的金属-酚类材料治疗剂，具有良好的预后和临床转化的潜力。
