Department of Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Raya Cisauk Lapan, Tangerang, Banten, 15345, Indonesia.
Department of Master in Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Jenderal Sudirman 51, Jakarta, 12930, Indonesia.
BMC Res Notes. 2024 Nov 14;17(1):338. doi: 10.1186/s13104-024-06997-0.
Food products are often contaminated by pathogens and spoilage bacteria. Most of them can form biofilms, a community of cells embedded in protective extracellular matrix layers resistant to harsh conditions, including antibiotics. Therefore, alternative antibiofilm agents are required to overcome biofilm formation. This study aims to determine and quantify the antibiofilm activity of supernatants from plant-associated bacteria against biofilms of foodborne pathogen and food spoilage bacterium, namely Bacillus cereus and Bacillus subtilis.
Plant-associated bacteria (PAB) have shown promising antibiofilm activities against biofilm-forming pathogens in previous studies. Thirteen PAB isolated from Ternate, Indonesia were used in this study. Supernatants of PAB were subjected to antimicrobial activity and quorum quenching detection, both using the well diffusion method. Four supernatants inhibited the growth of B. subtilis, but none affected the growth of B. cereus. Eight supernatants were able to disrupt the quorum sensing system of an indicator bacterium, wild-type Chromobacterium violaceum. Biofilm inhibition and destruction were quantified using 96-well microplates. The highest biofilm inhibition and destruction activities of PAB supernatants against each of B. cereus and B. subtilis biofilms were > 76%, and were later confirmed by light microscope and scanning electron microscope. Brine shrimp lethality assay (BSLA) was conducted and revealed that the selected PAB supernatants were non-toxic. The 16S rRNA gene of PAB were sequenced and they showed similarities to Bacillus, Priestia, and Chryseobacterium. Compounds in the supernatants were determined by GC-MS which revealed contents of fatty acids, ethyl esters, and diketopiperazines. Therefore, PAB supernatants have potential as antibiofilm agents against biofilm formed by Bacillus cereus and Bacillus subtilis.
食品经常受到病原体和腐败细菌的污染。它们中的大多数可以形成生物膜，这是一种由嵌入在保护性细胞外基质层中的细胞组成的群落，能够抵抗恶劣条件，包括抗生素。因此，需要替代的抗生物膜剂来克服生物膜的形成。本研究旨在确定和量化植物相关细菌（PAB）的上清液对食源性病原体和食源腐败细菌（即蜡状芽孢杆菌和枯草芽孢杆菌）生物膜的抗生物膜活性，并对其进行定量分析。
在之前的研究中，植物相关细菌（PAB）已显示出对生物膜形成病原体的有希望的抗生物膜活性。本研究使用了从印度尼西亚特尔纳特分离出的 13 株 PAB。使用平板孔扩散法对 PAB 的上清液进行了抗菌活性和群体感应淬灭检测。四种上清液抑制了枯草芽孢杆菌的生长，但没有一种上清液影响蜡状芽孢杆菌的生长。八种上清液能够破坏指示菌（野生型紫色色杆菌）的群体感应系统。使用 96 孔微孔板定量测定生物膜抑制和破坏。PAB 上清液对蜡状芽孢杆菌和枯草芽孢杆菌生物膜的最高生物膜抑制和破坏活性均>76%，并用荧光显微镜和扫描电子显微镜进一步证实。进行了卤虫致死试验（BSLA），结果表明所选 PAB 上清液无毒。对 PAB 的 16S rRNA 基因进行测序，结果表明它们与芽孢杆菌、普雷蒂亚菌和黄杆菌相似。通过 GC-MS 确定了上清液中的化合物，结果表明其中含有脂肪酸、乙酯和二酮哌嗪。因此，PAB 上清液具有作为抗生物膜剂抑制蜡状芽孢杆菌和枯草芽孢杆菌形成生物膜的潜力。
